TY - JOUR
T1 - A Transcriptomic Comparative Study of Cranial Vasculature
AU - GENIE Investigators
AU - Zhang, Jianing
AU - Ryu, Jee Yeon
AU - Tirado, Selena Rae
AU - Dickinson, Lawrence D.
AU - Abosch, Aviva
AU - Aziz-Sultan, M. Ali
AU - Boulos, Alan S.
AU - Barrow, Daniel L.
AU - Batjer, H. Hunt
AU - Binyamin, Tamar R.
AU - Blackburn, Spiros L.
AU - Chang, Edward F.
AU - Chen, P. Roc
AU - Colby, Geoffrey P.
AU - Cosgrove, G. Rees
AU - David, Carlos A.
AU - Day, Arthur L.
AU - Folkerth, Rebecca D.
AU - Frerichs, Kai U.
AU - Howard, Brian M.
AU - Jahromi, Behnam R.
AU - Niemela, Mika
AU - Ojemann, Steven G.
AU - Patel, Nirav J.
AU - Richardson, R. Mark
AU - Shi, Xiangen
AU - Valle-Giler, Edison P.
AU - Wang, Anthony C.
AU - Welch, Babu G.
AU - Williams, Ziv
AU - Zusman, Edie E.
AU - Weiss, Scott T.
AU - Du, Rose
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - In genetic studies of cerebrovascular diseases, the optimal vessels to use as controls remain unclear. Our goal is to compare the transcriptomic profiles among 3 different types of control vessels: superficial temporal artery (STA), middle cerebral arteries (MCA), and arteries from the circle of Willis obtained from autopsies (AU). We examined the transcriptomic profiles of STA, MCA, and AU using RNAseq. We also investigated the effects of using these control groups on the results of the comparisons between aneurysms and the control arteries. Our study showed that when comparing pathological cerebral arteries to control groups, all control groups presented similar responses in the activation of immunological processes, the regulation of intracellular signaling pathways, and extracellular matrix productions, despite their intrinsic biological differences. When compared to STA, AU exhibited upregulation of stress and apoptosis genes, whereas MCA showed upregulation of genes associated with tRNA/rRNA processing. Moreover, our results suggest that the matched case–control study design, which involves control STA samples collected from the same subjects of matched aneurysm samples in our study, can improve the identification of non-inherited disease-associated genes. Given the challenges associated with obtaining fresh intracranial arteries from healthy individuals, our study suggests that using MCA, AU, or paired STA samples as controls are feasible strategies for future large-scale studies investigating cerebral vasculopathies. However, the intrinsic differences of each type of control should be taken into consideration when interpreting the results. With the limitations of each control type, it may be most optimal to use multiple tissues as controls.
AB - In genetic studies of cerebrovascular diseases, the optimal vessels to use as controls remain unclear. Our goal is to compare the transcriptomic profiles among 3 different types of control vessels: superficial temporal artery (STA), middle cerebral arteries (MCA), and arteries from the circle of Willis obtained from autopsies (AU). We examined the transcriptomic profiles of STA, MCA, and AU using RNAseq. We also investigated the effects of using these control groups on the results of the comparisons between aneurysms and the control arteries. Our study showed that when comparing pathological cerebral arteries to control groups, all control groups presented similar responses in the activation of immunological processes, the regulation of intracellular signaling pathways, and extracellular matrix productions, despite their intrinsic biological differences. When compared to STA, AU exhibited upregulation of stress and apoptosis genes, whereas MCA showed upregulation of genes associated with tRNA/rRNA processing. Moreover, our results suggest that the matched case–control study design, which involves control STA samples collected from the same subjects of matched aneurysm samples in our study, can improve the identification of non-inherited disease-associated genes. Given the challenges associated with obtaining fresh intracranial arteries from healthy individuals, our study suggests that using MCA, AU, or paired STA samples as controls are feasible strategies for future large-scale studies investigating cerebral vasculopathies. However, the intrinsic differences of each type of control should be taken into consideration when interpreting the results. With the limitations of each control type, it may be most optimal to use multiple tissues as controls.
KW - Cerebral vasculature
KW - Intracerebral artery
KW - Intracranial aneurysm
KW - Superficial temporal artery
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U2 - 10.1007/s12975-023-01186-w
DO - 10.1007/s12975-023-01186-w
M3 - Article
C2 - 37612482
AN - SCOPUS:85169123174
SN - 1868-4483
JO - Translational Stroke Research
JF - Translational Stroke Research
ER -