TY - JOUR
T1 - A strategy for founding a global lymphocyte proteinpaedia and gene catalogue
AU - Lefkovits, Ivan
AU - Kettman, John R.
AU - Coleclough, Christopher
N1 - Funding Information:
We are indebted to Nlels K. Jerne and Sigmund Weiss for critical reading of the manuscript, and to Andre Traunecker for valuable discussions. We thank Large Scale Biology, Rockville, Maryland, for help in the computer image analyses, Janette Mdlar for expert preparation of the manuscript and Hanspeter Stahlberger for the artwork. Work performed at St Jude's Hospital was supported by grant CA21765 and the American Lebanese Syrian Associated Charities (ALSAC). The Basel Institute for Immunology was founded and is supported by F Hoffmann-La Roche and Co. Ltd, Basel, Switzerland.
PY - 1990
Y1 - 1990
N2 - In this article, Ivan Lefkovits, John Kettman and Christopher Coleclough describe a strategy for a comprehensive analysis of the genes active in lymphocytes. It allows direct access to the coding sequence of any gene, provided that the two-dimensional gel spot coordinate position is known. The authors have constructed representative lymphocyte cDNA libraries and partitioned them into many sectors, each containing a unique set of cDNA clones. Data from the analysis of natural lymphocyte protein is compiled into a 'proteinpaedia', which is matched to a gene catalogue comprising the partitioned recombinant phage collection.
AB - In this article, Ivan Lefkovits, John Kettman and Christopher Coleclough describe a strategy for a comprehensive analysis of the genes active in lymphocytes. It allows direct access to the coding sequence of any gene, provided that the two-dimensional gel spot coordinate position is known. The authors have constructed representative lymphocyte cDNA libraries and partitioned them into many sectors, each containing a unique set of cDNA clones. Data from the analysis of natural lymphocyte protein is compiled into a 'proteinpaedia', which is matched to a gene catalogue comprising the partitioned recombinant phage collection.
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U2 - 10.1016/0167-5699(90)90066-I
DO - 10.1016/0167-5699(90)90066-I
M3 - Article
C2 - 2186749
AN - SCOPUS:0025230843
SN - 1471-4906
VL - 11
SP - 157
EP - 162
JO - Trends in Immunology
JF - Trends in Immunology
IS - C
ER -