TY - JOUR
T1 - A single gene encodes soluble and membrane-bound forms of the major histocompatibility Qa-2 antigen
T2 - Anchoring of the product by a phospholipid tail
AU - Stroynowski, Iwona
AU - Soloski, Mark
AU - Low, Martin G.
AU - Hood, Leroy
N1 - Funding Information:
We thank K. Blackburn, professional assistance, clones encoding C57BUlO A. Lattimore. and K. Lewis for excellent Drs. G. Waneck and R. Flavell for DNA Qa-2 genes, Drs. I. McKenzie, D. Murphy, T. Hansen, and K. Ozato for gifts of antibody preparations, and Dr. L. Hoopes for help in setting up two-dimensional gel analysis. This work was supported by NIH grant R2320922 and ACS grant lM376 to M. Soloski and by NIH grant Al 19624 to L. Hood.
PY - 1987/8/28
Y1 - 1987/8/28
N2 - The H-2, Qa, and Tla genes of the murine major histocompatibility complex are related to each other by DNA sequence homology. The H-2 genes encode ubiquitously expressed transplantation antigens that serve as recognition structures for cytotoxic T cells. The identities of the Qa and Tla products, their sites of expression, and their functions are largely unknown. We report here that the Qa region gene Q7 encodes a membrane-bound as well as a secreted form of the serologically defined antigen Qa-2. The Q7 gene introduced into liver-derived cells is expressed as a membrane-bound and as a secreted molecule. In transfected L cells it is expressed only as a soluble protein. Biochemical analysis suggests that the Q7 product is anchored to the liver cell membranes by a phospholipid tail. This feature may be responsible for cell type-specific expression of the two forms of the Qa-2 molecules.
AB - The H-2, Qa, and Tla genes of the murine major histocompatibility complex are related to each other by DNA sequence homology. The H-2 genes encode ubiquitously expressed transplantation antigens that serve as recognition structures for cytotoxic T cells. The identities of the Qa and Tla products, their sites of expression, and their functions are largely unknown. We report here that the Qa region gene Q7 encodes a membrane-bound as well as a secreted form of the serologically defined antigen Qa-2. The Q7 gene introduced into liver-derived cells is expressed as a membrane-bound and as a secreted molecule. In transfected L cells it is expressed only as a soluble protein. Biochemical analysis suggests that the Q7 product is anchored to the liver cell membranes by a phospholipid tail. This feature may be responsible for cell type-specific expression of the two forms of the Qa-2 molecules.
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U2 - 10.1016/0092-8674(87)90334-5
DO - 10.1016/0092-8674(87)90334-5
M3 - Article
C2 - 2441874
AN - SCOPUS:0023667015
SN - 0092-8674
VL - 50
SP - 759
EP - 768
JO - Cell
JF - Cell
IS - 5
ER -