A Role for Nr-CAM in the Patterning of Binocular Visual Pathways

Scott E. Williams; Martin Grumet; David R. Colman; Mark Henkemeyer; Carol A. Mason; Takeshi Sakurai

doi:10.1016/j.neuron.2006.03.037

A Role for Nr-CAM in the Patterning of Binocular Visual Pathways

Scott E. Williams, Martin Grumet, David R. Colman, Mark Henkemeyer, Carol A. Mason, Takeshi Sakurai

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

Retinal ganglion cell (RGC) axons diverge within the optic chiasm to project to opposite sides of the brain. In mouse, contralateral RGCs are distributed throughout the retina, whereas ipsilateral RGCs are restricted to the ventrotemporal crescent (VTC). While repulsive guidance mechanisms play a major role in the formation of the ipsilateral projection, little is known about the contribution of growth-promoting interactions to the formation of binocular visual projections. Here, we show that the cell adhesion molecule Nr-CAM is expressed by RGCs that project contralaterally and is critical for the guidance of late-born RGCs within the VTC. Blocking Nr-CAM function causes an increase in the size of the ipsilateral projection and reduces neurite outgrowth on chiasm cells in an age- and region-specific manner. Finally, we demonstrate that EphB1/ephrin-B2-mediated repulsion and Nr-CAM-mediated attraction comprise distinct molecular programs that each contributes to the proper formation of binocular visual pathways.

Original language	English (US)
Pages (from-to)	535-547
Number of pages	13
Journal	Neuron
Volume	50
Issue number	4
DOIs	https://doi.org/10.1016/j.neuron.2006.03.037
State	Published - May 18 2006

Keywords

CELLBIO
DEVBIO
MOLNEURO

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/j.neuron.2006.03.037

Cite this

@article{39730a55489f4b5cb6cc9ecc7fbfaf2d,

title = "A Role for Nr-CAM in the Patterning of Binocular Visual Pathways",

abstract = "Retinal ganglion cell (RGC) axons diverge within the optic chiasm to project to opposite sides of the brain. In mouse, contralateral RGCs are distributed throughout the retina, whereas ipsilateral RGCs are restricted to the ventrotemporal crescent (VTC). While repulsive guidance mechanisms play a major role in the formation of the ipsilateral projection, little is known about the contribution of growth-promoting interactions to the formation of binocular visual projections. Here, we show that the cell adhesion molecule Nr-CAM is expressed by RGCs that project contralaterally and is critical for the guidance of late-born RGCs within the VTC. Blocking Nr-CAM function causes an increase in the size of the ipsilateral projection and reduces neurite outgrowth on chiasm cells in an age- and region-specific manner. Finally, we demonstrate that EphB1/ephrin-B2-mediated repulsion and Nr-CAM-mediated attraction comprise distinct molecular programs that each contributes to the proper formation of binocular visual pathways.",

keywords = "CELLBIO, DEVBIO, MOLNEURO",

author = "Williams, {Scott E.} and Martin Grumet and Colman, {David R.} and Mark Henkemeyer and Mason, {Carol A.} and Takeshi Sakurai",

note = "Funding Information: This work was supported by NIH grants R01 EY012736 and R01 EY015290 to C.A.M. and NINDS NS040560 and National Multiple Sclerosis Society RG3217-A-8 grants to D.R.C. S.E.W. was supported by NIH training grant T32 EY013933. T.S. is a Seaver Fellow. We are grateful to Nicholas Betley for the L1 and contactin family in situ probes, Steve Brown and Lucia Brown for the Zic2 antibody, Nathaniel Heintz and Shiaoching Gong for help with generating BAC transgenics, Susan Morton for TAG-1, RC2, and neurofilament antibodies, Peter Brophy for the Neurofascin antibody, Jim Salzer for the contactin antibody, and Joanne Babiarz, Hranush Melikyan, and Richard Blazeski for technical assistance. We are also grateful to Zaven Kaprielian, Lynda Erskine, Tom Jessell, Eloisa Herrera, Timothy Petros, Alexandra Rebsam, Amy Chung, Ramee Lee, and Andrew Tomlinson for helpful discussions and comments on the manuscript. ",

year = "2006",

month = may,

day = "18",

doi = "10.1016/j.neuron.2006.03.037",

language = "English (US)",

volume = "50",

pages = "535--547",

journal = "Neuron",

issn = "0896-6273",

publisher = "Cell Press",

number = "4",

}

TY - JOUR

T1 - A Role for Nr-CAM in the Patterning of Binocular Visual Pathways

AU - Williams, Scott E.

AU - Grumet, Martin

AU - Colman, David R.

AU - Henkemeyer, Mark

AU - Mason, Carol A.

AU - Sakurai, Takeshi

N1 - Funding Information: This work was supported by NIH grants R01 EY012736 and R01 EY015290 to C.A.M. and NINDS NS040560 and National Multiple Sclerosis Society RG3217-A-8 grants to D.R.C. S.E.W. was supported by NIH training grant T32 EY013933. T.S. is a Seaver Fellow. We are grateful to Nicholas Betley for the L1 and contactin family in situ probes, Steve Brown and Lucia Brown for the Zic2 antibody, Nathaniel Heintz and Shiaoching Gong for help with generating BAC transgenics, Susan Morton for TAG-1, RC2, and neurofilament antibodies, Peter Brophy for the Neurofascin antibody, Jim Salzer for the contactin antibody, and Joanne Babiarz, Hranush Melikyan, and Richard Blazeski for technical assistance. We are also grateful to Zaven Kaprielian, Lynda Erskine, Tom Jessell, Eloisa Herrera, Timothy Petros, Alexandra Rebsam, Amy Chung, Ramee Lee, and Andrew Tomlinson for helpful discussions and comments on the manuscript.

PY - 2006/5/18

Y1 - 2006/5/18

N2 - Retinal ganglion cell (RGC) axons diverge within the optic chiasm to project to opposite sides of the brain. In mouse, contralateral RGCs are distributed throughout the retina, whereas ipsilateral RGCs are restricted to the ventrotemporal crescent (VTC). While repulsive guidance mechanisms play a major role in the formation of the ipsilateral projection, little is known about the contribution of growth-promoting interactions to the formation of binocular visual projections. Here, we show that the cell adhesion molecule Nr-CAM is expressed by RGCs that project contralaterally and is critical for the guidance of late-born RGCs within the VTC. Blocking Nr-CAM function causes an increase in the size of the ipsilateral projection and reduces neurite outgrowth on chiasm cells in an age- and region-specific manner. Finally, we demonstrate that EphB1/ephrin-B2-mediated repulsion and Nr-CAM-mediated attraction comprise distinct molecular programs that each contributes to the proper formation of binocular visual pathways.

AB - Retinal ganglion cell (RGC) axons diverge within the optic chiasm to project to opposite sides of the brain. In mouse, contralateral RGCs are distributed throughout the retina, whereas ipsilateral RGCs are restricted to the ventrotemporal crescent (VTC). While repulsive guidance mechanisms play a major role in the formation of the ipsilateral projection, little is known about the contribution of growth-promoting interactions to the formation of binocular visual projections. Here, we show that the cell adhesion molecule Nr-CAM is expressed by RGCs that project contralaterally and is critical for the guidance of late-born RGCs within the VTC. Blocking Nr-CAM function causes an increase in the size of the ipsilateral projection and reduces neurite outgrowth on chiasm cells in an age- and region-specific manner. Finally, we demonstrate that EphB1/ephrin-B2-mediated repulsion and Nr-CAM-mediated attraction comprise distinct molecular programs that each contributes to the proper formation of binocular visual pathways.

KW - CELLBIO

KW - DEVBIO

KW - MOLNEURO

UR - http://www.scopus.com/inward/record.url?scp=33646418684&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646418684&partnerID=8YFLogxK

U2 - 10.1016/j.neuron.2006.03.037

DO - 10.1016/j.neuron.2006.03.037

M3 - Article

C2 - 16701205

AN - SCOPUS:33646418684

SN - 0896-6273

VL - 50

SP - 535

EP - 547

JO - Neuron

JF - Neuron

IS - 4

ER -

A Role for Nr-CAM in the Patterning of Binocular Visual Pathways

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this