TY - JOUR
T1 - A RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells
AU - Zawistowski, Jon S.
AU - Sabouri-Ghomi, Mohsen
AU - Danuser, Gaudenz
AU - Hahn, Klaus M.
AU - Hodgson, Louis
PY - 2013/11/5
Y1 - 2013/11/5
N2 - RhoA and RhoC GTPases share 92% amino acid sequence identity, yet play different roles in regulating cell motility and morphology. To understand these differences, we developed and validated a biosensor of RhoC activation (RhoC FLARE). This was used together with a RhoA biosensor to compare the spatio-temporal dynamics of RhoA and RhoC activity during cell protrusion/retraction and macropinocytosis. Both GTPases were activated similarly at the cell edge, but in regions more distal from the edge RhoC showed higher activation during protrusion. The two isoforms differed markedly in the kinetics of activation. RhoC was activated concomitantly with RhoA at the cell edge, but distally, RhoC activation preceded RhoA activation, occurring before edge protrusion. During macropinocytosis, differences were observed during vesicle closure and in the area surrounding vesicle formation.
AB - RhoA and RhoC GTPases share 92% amino acid sequence identity, yet play different roles in regulating cell motility and morphology. To understand these differences, we developed and validated a biosensor of RhoC activation (RhoC FLARE). This was used together with a RhoA biosensor to compare the spatio-temporal dynamics of RhoA and RhoC activity during cell protrusion/retraction and macropinocytosis. Both GTPases were activated similarly at the cell edge, but in regions more distal from the edge RhoC showed higher activation during protrusion. The two isoforms differed markedly in the kinetics of activation. RhoC was activated concomitantly with RhoA at the cell edge, but distally, RhoC activation preceded RhoA activation, occurring before edge protrusion. During macropinocytosis, differences were observed during vesicle closure and in the area surrounding vesicle formation.
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U2 - 10.1371/journal.pone.0079877
DO - 10.1371/journal.pone.0079877
M3 - Article
C2 - 24224016
AN - SCOPUS:84892419336
SN - 1932-6203
VL - 8
JO - PloS one
JF - PloS one
IS - 11
M1 - e79877
ER -