@article{91c8aa473ba143078dd9f8632050ec41,
title = "A protein-interaction network of interferon-stimulated genes extends the innate immune system landscape",
abstract = "Interferon-stimulated genes (ISGs) form the backbone of the innate immune system and are important for limiting intra- and intercellular viral replication and spread. We conducted a mass-spectrometry-based survey to understand the fundamental organization of the innate immune system and to explore the molecular functions of individual ISGs. We identified interactions between 104 ISGs and 1,401 cellular binding partners engaging in 2,734 high-confidence interactions. 90% of these interactions are unreported so far, and our survey therefore illuminates a far wider activity spectrum of ISGs than is currently known. Integration of the resulting ISG-interaction network with published datasets and functional studies allowed us to identify regulators of immunity and processes related to the immune system. Given the extraordinary robustness of the innate immune system, this ISG network may serve as a blueprint for therapeutic targeting of cellular systems to efficiently fight viral infections.",
author = "Philipp Hubel and Christian Urban and Valter Bergant and Schneider, {William M.} and Barbara Knauer and Alexey Stukalov and Pietro Scaturro and Angelika Mann and Linda Brunotte and Hoffmann, {Heinrich H.} and Schoggins, {John W.} and Martin Schwemmle and Matthias Mann and Rice, {Charles M.} and Andreas Pichlmair",
note = "Funding Information: We acknowledge the innate immunity laboratory for critical discussions and suggestions. We thank K. Mayr, I. Paron and G. Sowa for maintaining mass spectrometers and the MPI-B core facility for support. We further thank G. Kochs for the FluAV mini replicon and A549 cells, G. Zimmer for VSV-Luc and A. Merits for SFV-gLuc and SFV-NanoLuc. We also thank M. Feulner for technical assistance. The work in the authors' laboratories was supported by the Max-Planck Free Floater program, ERC starting grant (ERC-StG iVIP, 311339), ERC consolidator grant (ERC-CoG ProDAP, 817798), Infect-Era and the German Federal Ministry of Education and Research (ERASE), the German Research Foundation (PI 1084/2, PI 1084/3, P1083/4 TRR179 and TRR237) to A.P. and (MS 632/15) to M.S. This work was also supported by NIH grant R01-AI091707 (to C.M.R.). Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2019",
month = apr,
day = "1",
doi = "10.1038/s41590-019-0323-3",
language = "English (US)",
volume = "20",
pages = "493--502",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "Nature Publishing Group",
number = "4",
}