Abstract
We examined how a p53 enhancer transmits regulatory information in vivo. Using genetic ablation together with digital chromosome conformation capture and fluorescent in situ hybridization, we found that a Drosophila p53 enhancer region (referred to as the p53 response element [p53RE]) physically contacts targets in cis and across the centromere to control stress-responsive transcription at these sites. Furthermore, when placed at ectopic genomic positions, fragments spanning this element re-established chromatin contacts and partially restored target gene regulation to mutants lacking the native p53RE. Therefore, a defined p53 enhancer region is sufficient for longrange chromatin interactions that enable multigenic regulation.
Original language | English (US) |
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Pages (from-to) | 2433-2438 |
Number of pages | 6 |
Journal | Genes and Development |
Volume | 27 |
Issue number | 22 |
DOIs | |
State | Published - Nov 15 2013 |
Keywords
- Apoptosis
- Chromosome conformation capture
- Genome architecture
- Long-distance gene regulation
- P53
ASJC Scopus subject areas
- Genetics
- Developmental Biology