Abstract
A composite EBV-bssed expression vector, pEBAK, was constructed with the 5′-flanking sequence of human α-fetoprotein gene as a promoter. Completed to galactosylated histone, this vector with a foreign DNA insertion could be transferred into hepatic cells via the asialoglycoprotein receptor mediated endocytosis pathway. It was replicated as an episome, and its expression was restricted to the AFP positive hepatoma cells. This new gene delivery method has the following advantages: (i) absence of a potential toxicity is related to viral vectors; (ii) the targeting is specific to AFP positive hepatoma cells; (iii) the introduction of the EBV replicon into this system results in the high-level expression and long-term maintenance of the transferred gene. This novel gene delivery system has potential applications in gene therapy for the treatment of hepatocellular carcinoma.
Original language | English (US) |
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Pages (from-to) | 80-86 |
Number of pages | 7 |
Journal | Science in China, Series C: Life Sciences |
Volume | 41 |
Issue number | 1 |
DOIs | |
State | Published - 1998 |
Keywords
- AFP promoter
- EBV replicon vector
- Hepatoma cell
- Receptor-mediated gene transfer
ASJC Scopus subject areas
- General Energy
- General Environmental Science
- Energy Engineering and Power Technology
- General Biochemistry, Genetics and Molecular Biology
- General Agricultural and Biological Sciences
- Management, Monitoring, Policy and Law
- Fuel Technology
- Renewable Energy, Sustainability and the Environment
- Modeling and Simulation