TY - JOUR
T1 - A novel 2-hour method for rapid preparation of permanent paraffin sections when treating melanoma in situ with mohs micrographic surgery
AU - Mallipeddi, Raj
AU - Stark, Jeff
AU - Xie, Xian Jin
AU - Matthews, Mark
AU - Taylor, R. Stan
PY - 2008/11
Y1 - 2008/11
N2 - BACKGROUND Distinguishing sun-induced melanocyte atypia from residual melanoma in situ (MIS) can be challenging, particularly when working with frozen sections. Immunostains such as melanoma-associated antigen recognized by T cells (MART-1) can assist, but paraffin sections provide an optimal means of analyzing melanocyte morphology. OBJECTIVE To verify the effectiveness of a 2-hour paraffin processing technique that uses microwave technology in the preparation of MIS sections. METHODS Twelve MIS debulk specimens were divided into 4 pieces with each piece processed 1 of 4 ways: our 2-hour paraffin technique with hematoxylin and eosin (H&E), conventional 24-hour paraffin processing with H&E, frozen sections with H&E, and frozen sections with MART-1 immunostaining. A Mohs surgeon and a dermatopathologist compared all specimens in a blinded fashion using a 3-point ranking scale to assess ease of visualizing normal melanocytes, ease of visualizing abnormal melanocytes, and overall ability to adequately visualize epidermal and dermal structures. RESULTS A nonparametric signed rank test indicated no significant differences between our microwave technique and conventional paraffin processing in all 3 criteria (p=.29,.63,.75, respectively). Our microwave technique was significantly better than frozen H&E sections for all 3 criteria (p=.046,.004,.005, respectively). CONCLUSION This rapid microwave tissue processing technique is comparable with conventional paraffin section processing. The authors have indicated no significant interest with commercial supporters.
AB - BACKGROUND Distinguishing sun-induced melanocyte atypia from residual melanoma in situ (MIS) can be challenging, particularly when working with frozen sections. Immunostains such as melanoma-associated antigen recognized by T cells (MART-1) can assist, but paraffin sections provide an optimal means of analyzing melanocyte morphology. OBJECTIVE To verify the effectiveness of a 2-hour paraffin processing technique that uses microwave technology in the preparation of MIS sections. METHODS Twelve MIS debulk specimens were divided into 4 pieces with each piece processed 1 of 4 ways: our 2-hour paraffin technique with hematoxylin and eosin (H&E), conventional 24-hour paraffin processing with H&E, frozen sections with H&E, and frozen sections with MART-1 immunostaining. A Mohs surgeon and a dermatopathologist compared all specimens in a blinded fashion using a 3-point ranking scale to assess ease of visualizing normal melanocytes, ease of visualizing abnormal melanocytes, and overall ability to adequately visualize epidermal and dermal structures. RESULTS A nonparametric signed rank test indicated no significant differences between our microwave technique and conventional paraffin processing in all 3 criteria (p=.29,.63,.75, respectively). Our microwave technique was significantly better than frozen H&E sections for all 3 criteria (p=.046,.004,.005, respectively). CONCLUSION This rapid microwave tissue processing technique is comparable with conventional paraffin section processing. The authors have indicated no significant interest with commercial supporters.
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U2 - 10.1111/j.1524-4725.2008.34316.x
DO - 10.1111/j.1524-4725.2008.34316.x
M3 - Article
C2 - 18811716
AN - SCOPUS:55949108689
SN - 1076-0512
VL - 34
SP - 1520
EP - 1526
JO - Dermatologic Surgery
JF - Dermatologic Surgery
IS - 11
ER -