TY - JOUR
T1 - A monoclonal antibody against a new differentiation antigen of thymocytes
AU - Sidman, C. L.
AU - Forni, L.
AU - Kohler, G.
AU - Langhorne, J.
AU - Lindahl, K. F.
PY - 1983
Y1 - 1983
N2 - B14−2−14 is a monoclonal cytotoxic IgM antibody which reacts with thymocytes of all mouse strains tested. The fraction of positive cells (by visual immunofluorescence) varies between strains from about 25–45% in A. CA to 65–85% in C57BL/6, and high levels are dominant in F1 hybrids. In the periphery, the antigen is found on a few percent of lymph node and not on splenic T cells, and it is absent in nude mice. Among thymocytes, the distribution of the B14 determinant largely overlaps with that of the TL antigen and of molecules binding peanut agglutinin. The B14 antibody reacts only minimally with hydrocortisone‐resistant thymus cells. Biochemical analysis shows that B14 antibody, anti‐TL antibody and peanut agglutinin bind to separate molecules. The target of the B14 antibody may be either an immature, thymic form of Thy‐1, or another molecule associated with it. Two polypeptides, of 40 and 35 kDa are precipitated by both B14 and anti‐Thy‐1 antibodies from biosynthetically labeled thymus cell lysates, and two others, of 27 and 17 kDa, from surface‐iodinated thymus cell preparations. B14‐2‐14 offers an additional method for identification and selection of thymocytes at different stages of differentiation, and should also be useful for studies of the Thy‐1 antigen.
AB - B14−2−14 is a monoclonal cytotoxic IgM antibody which reacts with thymocytes of all mouse strains tested. The fraction of positive cells (by visual immunofluorescence) varies between strains from about 25–45% in A. CA to 65–85% in C57BL/6, and high levels are dominant in F1 hybrids. In the periphery, the antigen is found on a few percent of lymph node and not on splenic T cells, and it is absent in nude mice. Among thymocytes, the distribution of the B14 determinant largely overlaps with that of the TL antigen and of molecules binding peanut agglutinin. The B14 antibody reacts only minimally with hydrocortisone‐resistant thymus cells. Biochemical analysis shows that B14 antibody, anti‐TL antibody and peanut agglutinin bind to separate molecules. The target of the B14 antibody may be either an immature, thymic form of Thy‐1, or another molecule associated with it. Two polypeptides, of 40 and 35 kDa are precipitated by both B14 and anti‐Thy‐1 antibodies from biosynthetically labeled thymus cell lysates, and two others, of 27 and 17 kDa, from surface‐iodinated thymus cell preparations. B14‐2‐14 offers an additional method for identification and selection of thymocytes at different stages of differentiation, and should also be useful for studies of the Thy‐1 antigen.
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U2 - 10.1002/eji.1830130610
DO - 10.1002/eji.1830130610
M3 - Article
C2 - 6407850
AN - SCOPUS:0020525995
SN - 0014-2980
VL - 13
SP - 481
EP - 488
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 6
ER -