A live-imaging protocol for tracking intestinal stem cell divisions in the Drosophila melanogaster pupal midgut

Song Wu; Ruizhi Tang; Benjamin Ohlstein; Zheng Guo

doi:10.1016/j.xpro.2023.102749

A live-imaging protocol for tracking intestinal stem cell divisions in the Drosophila melanogaster pupal midgut

Song Wu, Ruizhi Tang, Benjamin Ohlstein, Zheng Guo

Research output: Contribution to journal › Article › peer-review

Abstract

Establishing a long-term ex vivo observation of the intestinal stem cell (ISC) is crucial to help understand the formation and homeostasis of the intestinal epithelium. Here, we present a protocol for tracking the division of Drosophila pupal ISCs during pupal midgut development. We describe steps for dissecting, mounting, and live imaging the pupal midgut. We then detail procedures for fluorescence quantification of each cell. This protocol can be applied to other fluorescently tagged proteins. For complete details on the use and execution of this protocol, please refer to Wu et al.¹

Original language	English (US)
Article number	102749
Journal	STAR Protocols
Volume	4
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2023.102749
State	Published - Dec 15 2023

Keywords

Cell Biology
Cell culture
Developmental biology

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2023.102749

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abstract = "Establishing a long-term ex vivo observation of the intestinal stem cell (ISC) is crucial to help understand the formation and homeostasis of the intestinal epithelium. Here, we present a protocol for tracking the division of Drosophila pupal ISCs during pupal midgut development. We describe steps for dissecting, mounting, and live imaging the pupal midgut. We then detail procedures for fluorescence quantification of each cell. This protocol can be applied to other fluorescently tagged proteins. For complete details on the use and execution of this protocol, please refer to Wu et al.1",

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AU - Wu, Song

AU - Tang, Ruizhi

AU - Ohlstein, Benjamin

AU - Guo, Zheng

PY - 2023/12/15

Y1 - 2023/12/15

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AB - Establishing a long-term ex vivo observation of the intestinal stem cell (ISC) is crucial to help understand the formation and homeostasis of the intestinal epithelium. Here, we present a protocol for tracking the division of Drosophila pupal ISCs during pupal midgut development. We describe steps for dissecting, mounting, and live imaging the pupal midgut. We then detail procedures for fluorescence quantification of each cell. This protocol can be applied to other fluorescently tagged proteins. For complete details on the use and execution of this protocol, please refer to Wu et al.1

KW - Cell Biology

KW - Cell culture

KW - Developmental biology

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A live-imaging protocol for tracking intestinal stem cell divisions in the Drosophila melanogaster pupal midgut

Abstract

Keywords

ASJC Scopus subject areas

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