@article{f34e3c1048774340a51337df98d4a5fe,
title = "Yeast Ataxin-2 Forms an Intracellular Condensate Required for the Inhibition of TORC1 Signaling during Respiratory Growth",
abstract = "Unpacking the role of yeast ataxin-2 reveals that it preserves mitochondrial homeostasis, undergoing a condition-sensitive phase transition to modulate TORC1 function.",
keywords = "Ataxin-2, Autophagy, Low-complexity sequence, Pbp1, TORC1, cross-β, gel, mitochondria, phase separation, polymer, respiration",
author = "Yang, {Yu San} and Masato Kato and Xi Wu and Athanasios Litsios and Sutter, {Benjamin M.} and Yun Wang and Hsu, {Chien Hsiang} and Wood, {N. Ezgi} and Andrew Lemoff and Hamid Mirzaei and Matthias Heinemann and Tu, {Benjamin P.}",
note = "Funding Information: We thank K. Luby-Phelps and the UTSW Live Cell Imaging Core Facility for assistance with microscopy experiments. We thank S. McKnight, S. Fu, Y. Kuo, and the Tu Lab for valuable advice and suggestions. B.P.T. is supported by grants from the NIH (R01GM094314), the Welch Foundation (I-1797), the David and Lucile Packard Foundation, and a HHMI-Simons Faculty Scholars Award. M.K. is funded by the support of an anonymous donor to the laboratory of S. McKnight. N.E.W. is supported by Cancer Prevention and Research Institute of Texas (RR150058) and Welch Foundation (I-1919), which were obtained by A. Doncic. This study was conceived by Y.-S.Y. and B.P.T. M.K. performed in vitro assays of phase-separated droplet formation. X.W. originally identified Pbp1 in a visual screen for novel autophagy regulators. A. Litsios and M.H. performed microfluidic imaging and quantitative analysis of cell divisions. A. Lemoff and H.M. performed SILAC analysis. B.M.S. and Y.W. assisted with mutant and strain construction. C.-H.H. assisted with the computational analysis of SILAC proteome data and methionine content. N.E.W. performed image processing analysis of Pbp1 distribution. Y.-S.Y. led and performed all other experiments. The paper was written by Y.-S.Y. and B.P.T. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = apr,
day = "18",
doi = "10.1016/j.cell.2019.02.043",
language = "English (US)",
volume = "177",
pages = "697--710.e17",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",
}