XRCC3 is required for efficient repair of chromosome breaks by homologous recombination

Mark A. Brenneman; Anne E. Weiss; Jac A. Nickoloff; David J. Chen

doi:10.1016/S0921-8777(00)00002-1

XRCC3 is required for efficient repair of chromosome breaks by homologous recombination

Mark A. Brenneman, Anne E. Weiss, Jac A. Nickoloff, David J. Chen

Research output: Contribution to journal › Article › peer-review

159 Scopus citations

Abstract

XRCC3 was originally identified as a human gene able to complement the DNA damage sensitivity, chromosomal instability and impaired growth of the mutant hamster cell line irs1SF. More recently, it has been cloned, sequenced and found to bear sequence homology to the highly conserved eukaryotic repair and recombination gene RAD51. The phenotype of irs1SF and the identification of XRCC3 as a member of the RAD51 gene family have suggested a role for XRCC3 in repair of DNA damage by homologous recombination. Homologous recombinational repair (HRR) of a specifically induced chromosomal double-strand break (DSB) was assayed in irs1SF cells with and without transient complementation by human XRCC3. Complementation with XRCC3 increased the frequencies of repair by 34- to 260-fold. The results confirm a role for XRCC3 in HRR of DNA DSB, and the importance of this repair pathway for the maintenance of chromosomal integrity in mammalian cells. Copyright (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	89-97
Number of pages	9
Journal	Mutation Research - DNA Repair
Volume	459
Issue number	2
DOIs	https://doi.org/10.1016/S0921-8777(00)00002-1
State	Published - Mar 20 2000

Keywords

Chromosomal stability
Chromosome breaks
DNA double-strand breaks
DNA repair
Homologous recombination
XRCC3

ASJC Scopus subject areas

Molecular Biology
Toxicology
Genetics

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10.1016/S0921-8777(00)00002-1

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title = "XRCC3 is required for efficient repair of chromosome breaks by homologous recombination",

abstract = "XRCC3 was originally identified as a human gene able to complement the DNA damage sensitivity, chromosomal instability and impaired growth of the mutant hamster cell line irs1SF. More recently, it has been cloned, sequenced and found to bear sequence homology to the highly conserved eukaryotic repair and recombination gene RAD51. The phenotype of irs1SF and the identification of XRCC3 as a member of the RAD51 gene family have suggested a role for XRCC3 in repair of DNA damage by homologous recombination. Homologous recombinational repair (HRR) of a specifically induced chromosomal double-strand break (DSB) was assayed in irs1SF cells with and without transient complementation by human XRCC3. Complementation with XRCC3 increased the frequencies of repair by 34- to 260-fold. The results confirm a role for XRCC3 in HRR of DNA DSB, and the importance of this repair pathway for the maintenance of chromosomal integrity in mammalian cells. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Chromosomal stability, Chromosome breaks, DNA double-strand breaks, DNA repair, Homologous recombination, XRCC3",

author = "Brenneman, {Mark A.} and Weiss, {Anne E.} and Nickoloff, {Jac A.} and Chen, {David J.}",

note = "Funding Information: We thank Brant M. Wagener for expert technical support; Chris P. Allen for technical advice and critical discussions; Greg Donoho (Lexicon Genetics, The Woodlands, TX) for providing the I– Sce I expression vector pCMV(3×NLS)I– Sce I, and Nan Liu (Lawrence Livermore National Laboratory) for providing the human XRCC3 expression vector pREP– XRCC3 . We also thank Dr. Shunichi Takeda (Faculty of Medicine, Kyoto University, Japan) for discussing data prior to publication. This study was supported by the US Department of Energy and by an NIH grant (CA74046, D.J. Chen). ",

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doi = "10.1016/S0921-8777(00)00002-1",

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TY - JOUR

T1 - XRCC3 is required for efficient repair of chromosome breaks by homologous recombination

AU - Brenneman, Mark A.

AU - Weiss, Anne E.

AU - Nickoloff, Jac A.

AU - Chen, David J.

N1 - Funding Information: We thank Brant M. Wagener for expert technical support; Chris P. Allen for technical advice and critical discussions; Greg Donoho (Lexicon Genetics, The Woodlands, TX) for providing the I– Sce I expression vector pCMV(3×NLS)I– Sce I, and Nan Liu (Lawrence Livermore National Laboratory) for providing the human XRCC3 expression vector pREP– XRCC3 . We also thank Dr. Shunichi Takeda (Faculty of Medicine, Kyoto University, Japan) for discussing data prior to publication. This study was supported by the US Department of Energy and by an NIH grant (CA74046, D.J. Chen).

PY - 2000/3/20

Y1 - 2000/3/20

N2 - XRCC3 was originally identified as a human gene able to complement the DNA damage sensitivity, chromosomal instability and impaired growth of the mutant hamster cell line irs1SF. More recently, it has been cloned, sequenced and found to bear sequence homology to the highly conserved eukaryotic repair and recombination gene RAD51. The phenotype of irs1SF and the identification of XRCC3 as a member of the RAD51 gene family have suggested a role for XRCC3 in repair of DNA damage by homologous recombination. Homologous recombinational repair (HRR) of a specifically induced chromosomal double-strand break (DSB) was assayed in irs1SF cells with and without transient complementation by human XRCC3. Complementation with XRCC3 increased the frequencies of repair by 34- to 260-fold. The results confirm a role for XRCC3 in HRR of DNA DSB, and the importance of this repair pathway for the maintenance of chromosomal integrity in mammalian cells. Copyright (C) 2000 Elsevier Science B.V.

AB - XRCC3 was originally identified as a human gene able to complement the DNA damage sensitivity, chromosomal instability and impaired growth of the mutant hamster cell line irs1SF. More recently, it has been cloned, sequenced and found to bear sequence homology to the highly conserved eukaryotic repair and recombination gene RAD51. The phenotype of irs1SF and the identification of XRCC3 as a member of the RAD51 gene family have suggested a role for XRCC3 in repair of DNA damage by homologous recombination. Homologous recombinational repair (HRR) of a specifically induced chromosomal double-strand break (DSB) was assayed in irs1SF cells with and without transient complementation by human XRCC3. Complementation with XRCC3 increased the frequencies of repair by 34- to 260-fold. The results confirm a role for XRCC3 in HRR of DNA DSB, and the importance of this repair pathway for the maintenance of chromosomal integrity in mammalian cells. Copyright (C) 2000 Elsevier Science B.V.

KW - Chromosomal stability

KW - Chromosome breaks

KW - DNA double-strand breaks

KW - DNA repair

KW - Homologous recombination

KW - XRCC3

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ER -

XRCC3 is required for efficient repair of chromosome breaks by homologous recombination

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