@article{f5cbc941fd5e4ef6b6c39b28f674be0a,
title = "WNK1 kinase balances T cell adhesion versus migration in vivo",
abstract = "Adhesion and migration of T cells are controlled by chemokines and by adhesion molecules, especially integrins, and have critical roles in the normal physiological function of T lymphocytes. Using an RNA-mediated interference screen, we identified the WNK1 kinase as a regulator of both integrin-mediated adhesion and T cell migration. We found that WNK1 is a negative regulator of integrin-mediated adhesion, whereas it acts as a positive regulator of migration via the kinases OXSR1 and STK39 and the ion co-transporter SLC12A2. WNK1-deficient T cells home less efficiently to lymphoid organs and migrate more slowly through them. Our results reveal that a pathway previously known only to regulate salt homeostasis in the kidney functions to balance T cell adhesion and migration.",
author = "Robert K{\"o}chl and Flavian Thelen and Lesley Vanes and Braz{\~a}o, {Tiago F.} and Kathryn Fountain and Jian Xie and Huang, {Chou Long} and Ruth Lyck and Stein, {Jens V.} and Tybulewicz, {Victor L J}",
note = "Funding Information: We thank D. Alessi (University of Dundee) for Oxsr1T185A mice, anti-pSer325-OXSR1 antibodies and discussions; R. Zamoyska (University of Edinburgh) for Jurkat cells, D. Bell (Francis Crick Institute) for help with image analysis; M. Abadier (Theodor Kocher Institute) for isolation and cultivation of primary mouse brain microvascular endothelial cells; N. Hogg (Francis Crick Institute) for Kim127 and m24 antibodies; and L. Satlin and C. Else (Mount Sinai Medical Center) for access to Slc12a2 mutant mice (supported by the NIH NIDDK grant P30 DK079307, Pittsburgh Center For Kidney Research, Core B). Supported by the Francis Crick Institute (V.T.), which receives its core funding from the Medical Research Council, Cancer Research UK and the Wellcome Trust, the Medical Research Council (U117527252 to V.T.), the Wellcome Trust (089185 to R.K. and V.T.), the Biotechnology and Biological Sciences Research Council (BB/L00805X/1 to R.K. and V.T.), the US National Institute of Health (DK59530 to C.-L.H.) and the Swiss Multiple Sclerosis Society (R.L.). Publisher Copyright: {\textcopyright} 2016 Nature America, Inc.",
year = "2016",
month = aug,
day = "19",
doi = "10.1038/ni.3495",
language = "English (US)",
volume = "17",
pages = "1075--1083",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "Nature Publishing Group",
number = "9",
}