TY - JOUR
T1 - Widespread distribution of orexin in rat brain and its regulation upon fasting
AU - Mondal, Muhtashan S.
AU - Nakazato, Masamitsu
AU - Date, Yukari
AU - Murakami, Noboru
AU - Yanagisawa, Masashi
AU - Matsukura, Shigeru
N1 - Funding Information:
We thank Dr. Hideki Yamaguchi and Mr. Takanori Ida for their technical assistance. This study was supported in part by grants from the Ministry of Education, Science, Sports and Culture, Japan and Chiyoda Mutual Life Foundation to M. N.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1999/3/24
Y1 - 1999/3/24
N2 - Orexins A and B, novel hypothalamic peptides encoded by a single mRNA transcript, stimulate food intake. Two antisera specific for the individual rat orexins were prepared and sensitive RIAs developed. Orexin-A and -B are abundant in the rat hypothalamus, medulla-pons, and midbrain-thalamus, and moderately abundant in the cerebral cortex. No orexins were found in the adipose tissues or visceral organs studied. The major endogenous molecule of orexin-A is a 33-amino-acid peptide, and that of orexin-B a 28-amino-acid peptide. After a 48 h fast, the orexin-A and -B contents of the lateral hypothalamus exhibited a trend to increase, but the contents of other brain tissues significantly decreased as compared with the fed control rats. No circadian variations in the orexin contents were found in the brain. The extensive and abundant distribution of orexins in the brain and changes in their contents upon fasting suggest that they serve as neuromodulators and/or neurotransmitters that regulate feeding behavior through interaction with diverse neural networks.
AB - Orexins A and B, novel hypothalamic peptides encoded by a single mRNA transcript, stimulate food intake. Two antisera specific for the individual rat orexins were prepared and sensitive RIAs developed. Orexin-A and -B are abundant in the rat hypothalamus, medulla-pons, and midbrain-thalamus, and moderately abundant in the cerebral cortex. No orexins were found in the adipose tissues or visceral organs studied. The major endogenous molecule of orexin-A is a 33-amino-acid peptide, and that of orexin-B a 28-amino-acid peptide. After a 48 h fast, the orexin-A and -B contents of the lateral hypothalamus exhibited a trend to increase, but the contents of other brain tissues significantly decreased as compared with the fed control rats. No circadian variations in the orexin contents were found in the brain. The extensive and abundant distribution of orexins in the brain and changes in their contents upon fasting suggest that they serve as neuromodulators and/or neurotransmitters that regulate feeding behavior through interaction with diverse neural networks.
UR - http://www.scopus.com/inward/record.url?scp=0033599557&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033599557&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.0362
DO - 10.1006/bbrc.1999.0362
M3 - Article
C2 - 10080926
AN - SCOPUS:0033599557
SN - 0006-291X
VL - 256
SP - 495
EP - 499
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -