@article{aa95982b889b4946849018f62d546813,
title = "Widely Distributed Neurotensinergic Neurons in the Brainstem Regulate NREM Sleep in Mice",
abstract = "Kashiwagi et al. identify a novel NREM sleep-promoting neural circuit distributed in the midbrain, pons, and medulla. All the neurons of this circuit are positive for the neuropeptide neurotensin, and neurotensin itself is involved in sleep regulation.",
keywords = "DREADD, Nts, SubLDT, brainstem, chemogenetics, mouse, neurotensin, sleep",
author = "Mitsuaki Kashiwagi and Mika Kanuka and Chika Tatsuzawa and Hitomi Suzuki and Miho Morita and Kaeko Tanaka and Taizo Kawano and Shin, {Jay W.} and Harukazu Suzuki and Shigeyoshi Itohara and Masashi Yanagisawa and Yu Hayashi",
note = "Funding Information: We thank T. Kanda for AAV packaging; T. Nakashiba for the GFP-T2A-TeTx sequence; P.R. Dobner, J. Li, and B.M. Evers for the Nts-knockout strain; B. Roth, Salk Vector Core, UNC Vector Core, and University of Pennsylvania Vector Core for plasmids and AAV. This work was supported by the JSPS KAKENHI under grant numbers JP17J00738 (to M. Kashiwagi) and JP16H06141 and JP16H01264 (to Y.H.), AMED under grant numbers JP19dm0107138 and JP19gm1110008 , JST under grant number JPMJPR13AC , the MEXT WPI program , the Cell Science Research Foundation , the Asahi Glass Foundation , the Nakajima Foundation , the Senri Life Science Foundation , the Kanae Foundation for the Promotion of Medical Science , the Takeda Science Foundation , the Mitsubishi Foundation , the Mochida Memorial Foundation for Medical and Pharmaceutical Research , the Astellas Foundation for Research on Metabolic Disorders , the Sumitomo Foundation , the SENSHIN Medical Research Foundation , and the Japan Foundation for Applied Enzymology (TMFC) (to Y.H.). Funding Information: We thank T. Kanda for AAV packaging; T. Nakashiba for the GFP-T2A-TeTx sequence; P.R. Dobner, J. Li, and B.M. Evers for the Nts-knockout strain; B. Roth, Salk Vector Core, UNC Vector Core, and University of Pennsylvania Vector Core for plasmids and AAV. This work was supported by the JSPS KAKENHI under grant numbers JP17J00738 (to M. Kashiwagi) and JP16H06141 and JP16H01264 (to Y.H.), AMED under grant numbers JP19dm0107138 and JP19gm1110008, JST under grant number JPMJPR13AC, the MEXT WPI program, the Cell Science Research Foundation, the Asahi Glass Foundation, the Nakajima Foundation, the Senri Life Science Foundation, the Kanae Foundation for the Promotion of Medical Science, the Takeda Science Foundation, the Mitsubishi Foundation, the Mochida Memorial Foundation for Medical and Pharmaceutical Research, the Astellas Foundation for Research on Metabolic Disorders, the Sumitomo Foundation, the SENSHIN Medical Research Foundation, and the Japan Foundation for Applied Enzymology (TMFC) (to Y.H.). Conceptualization, Supervision, and Project Administration, Y.H.; Investigation, M. Kashiwagi, M. Kanuka, C.T. Hitomi Suzuki, M.M. K.T. T.K. J.W.S. and Y.H.; Resources, Harukazu Suzuki, S.I. M.Y. and Y.H.; Writing ? Original Draft, M. Kashiwagi and Y.H.; Writing ? Review & Editing, M.Y, and Y.H. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",
year = "2020",
month = mar,
day = "23",
doi = "10.1016/j.cub.2020.01.047",
language = "English (US)",
volume = "30",
pages = "1002--1010.e4",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "6",
}