Visualization of individual axons in excised lamprey spinal cord by magnetic resonance microscopy

Alexander C. Wright; Suzanne L. Wehrli; Guixin Zhang; Masaya Takahashi; David B. Hackney; Michael E. Selzer; Felix W. Wehrli

doi:10.1016/S0165-0270(01)00503-9

Visualization of individual axons in excised lamprey spinal cord by magnetic resonance microscopy

Alexander C. Wright, Suzanne L. Wehrli, Guixin Zhang, Masaya Takahashi, David B. Hackney, Michael E. Selzer, Felix W. Wehrli

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The direct visualization of axons within their native tissue environment by magnetic resonance (MR) microscopy is presented for the first time in the excised larval sea lamprey spinal cord. A home-built transverse radio frequency coil of 1.5 mm diameter was used in conjunction with a commercial 400 MHz MR microscopy system, implementing both 2-D and 3-D imaging pulse sequences. Images having nominal voxel sizes of 9×9×500 and 9×9×125 μm³, respectively, in the lamprey spinal cord were obtained, resolving individual Mauthner and Müller axons. Furthermore, architectural changes associated with axonal degeneration were visualized in the spinal cord of one animal, excised 8 weeks after hemisection of the cord. Although the lamprey previously has not been the subject of MR microscopy investigations, these results demonstrate the method's potential for imaging this axon system, which is an important model of spinal cord injury and regeneration.

Original language	English (US)
Pages (from-to)	9-15
Number of pages	7
Journal	Journal of Neuroscience Methods
Volume	114
Issue number	1
DOIs	https://doi.org/10.1016/S0165-0270(01)00503-9
State	Published - Feb 15 2002

Keywords

Axons
Imaging
Lamprey
Magnetic resonance microscopy
Spinal cord injury

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0165-0270(01)00503-9

Cite this

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title = "Visualization of individual axons in excised lamprey spinal cord by magnetic resonance microscopy",

abstract = "The direct visualization of axons within their native tissue environment by magnetic resonance (MR) microscopy is presented for the first time in the excised larval sea lamprey spinal cord. A home-built transverse radio frequency coil of 1.5 mm diameter was used in conjunction with a commercial 400 MHz MR microscopy system, implementing both 2-D and 3-D imaging pulse sequences. Images having nominal voxel sizes of 9×9×500 and 9×9×125 μm3, respectively, in the lamprey spinal cord were obtained, resolving individual Mauthner and M{\"u}ller axons. Furthermore, architectural changes associated with axonal degeneration were visualized in the spinal cord of one animal, excised 8 weeks after hemisection of the cord. Although the lamprey previously has not been the subject of MR microscopy investigations, these results demonstrate the method's potential for imaging this axon system, which is an important model of spinal cord injury and regeneration.",

keywords = "Axons, Imaging, Lamprey, Magnetic resonance microscopy, Spinal cord injury",

author = "Wright, {Alexander C.} and Wehrli, {Suzanne L.} and Guixin Zhang and Masaya Takahashi and Hackney, {David B.} and Selzer, {Michael E.} and Wehrli, {Felix W.}",

note = "Funding Information: This research was supported by NIH grants R01 NS25921, R01 NS14837 and R01 NS25581.",

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AU - Wright, Alexander C.

AU - Wehrli, Suzanne L.

AU - Zhang, Guixin

AU - Takahashi, Masaya

AU - Hackney, David B.

AU - Selzer, Michael E.

AU - Wehrli, Felix W.

N1 - Funding Information: This research was supported by NIH grants R01 NS25921, R01 NS14837 and R01 NS25581.

PY - 2002/2/15

Y1 - 2002/2/15

N2 - The direct visualization of axons within their native tissue environment by magnetic resonance (MR) microscopy is presented for the first time in the excised larval sea lamprey spinal cord. A home-built transverse radio frequency coil of 1.5 mm diameter was used in conjunction with a commercial 400 MHz MR microscopy system, implementing both 2-D and 3-D imaging pulse sequences. Images having nominal voxel sizes of 9×9×500 and 9×9×125 μm3, respectively, in the lamprey spinal cord were obtained, resolving individual Mauthner and Müller axons. Furthermore, architectural changes associated with axonal degeneration were visualized in the spinal cord of one animal, excised 8 weeks after hemisection of the cord. Although the lamprey previously has not been the subject of MR microscopy investigations, these results demonstrate the method's potential for imaging this axon system, which is an important model of spinal cord injury and regeneration.

AB - The direct visualization of axons within their native tissue environment by magnetic resonance (MR) microscopy is presented for the first time in the excised larval sea lamprey spinal cord. A home-built transverse radio frequency coil of 1.5 mm diameter was used in conjunction with a commercial 400 MHz MR microscopy system, implementing both 2-D and 3-D imaging pulse sequences. Images having nominal voxel sizes of 9×9×500 and 9×9×125 μm3, respectively, in the lamprey spinal cord were obtained, resolving individual Mauthner and Müller axons. Furthermore, architectural changes associated with axonal degeneration were visualized in the spinal cord of one animal, excised 8 weeks after hemisection of the cord. Although the lamprey previously has not been the subject of MR microscopy investigations, these results demonstrate the method's potential for imaging this axon system, which is an important model of spinal cord injury and regeneration.

KW - Axons

KW - Imaging

KW - Lamprey

KW - Magnetic resonance microscopy

KW - Spinal cord injury

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Visualization of individual axons in excised lamprey spinal cord by magnetic resonance microscopy

Abstract

Keywords

ASJC Scopus subject areas

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