Using seahorse machine to measure OCR and ECAR in cancer cells

Jing Zhang, Qing Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

79 Scopus citations


A large amount of energy used for nutrient processing and cellular functions is essential for tumorigenesis. Total intracellular adenosine triphosphate (ATP) is mainly generated by glycolysis and mitochondrial oxidative phosphorylation. Here, we provide a protocol for measurements of energy metabolism in cancer cells by using Seahorse XF24 Extracellular Flux analyzer. Specifically, this machine measures glycolysis by analyzing the extracellular acidification rate (ECAR) and measures mitochondrial oxidative phosphorylation on the basis of the oxygen consumption rate (OCR), through real-time and live cell analysis. This protocol is provided for researchers who are unfamiliar with the method and to aid them in carrying out the technique successfully.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
StatePublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • ECAR
  • Energy metabolism
  • Glycolysis
  • OCR
  • Oxidative phosphorylation
  • Seahorse XF24 Extracellular Flux analyzer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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