TY - JOUR
T1 - Using micro-synchrotron radiation x-ray fluorescence (µ-SRXRF) for trace metal imaging in the development of MRI contrast agents for prostate cancer imaging
AU - Dao, E.
AU - Clavijo Jordan, M. V.
AU - Geraki, K.
AU - Martins, A. F.
AU - Chirayil, S.
AU - Sherry, A. D.
AU - Farquharson, M. J.
N1 - Funding Information:
Financial support from the National Institutes of Health, USA ( DK-095416 ), the Cancer Prevention and Research Institute of Texas, USA ( RP180178 ), and the Robert A. Welch Foundation, USA ( AT-584 ) are gratefully acknowledged. We also acknowledge Diamond Light Source, UK for beamtime on I18 under proposal sp23705–3.
Publisher Copyright:
© 2022 Elsevier GmbH
PY - 2022/12
Y1 - 2022/12
N2 - Background: Contrast agents (CA) are administered in magnetic resonance imaging (MRI) clinical exams to measure tissue perfusion, enhance image contrast between adjacent tissues, or provide additional biochemical information in molecular MRI. The efficacy of a CA is determined by the tissue distribution of the agent and its concentration in the extracellular space of all tissues. Methods: In this work, micro-synchrotron radiation x-ray fluorescence (µ-SRXRF) was used to examine and characterize a gadolinium-based zinc-sensitive agent (GdL2) currently under development for detection of prostate cancer (PCa) by MRI. Prostate tissue samples were collected from control mice and mice with known PCa after an MRI exam that included injection of GdL2. The samples were raster scanned to investigate trends in Zn, Gd, Cu, Fe, S, P, and Ca. Results: Significant Zn and Gd co-localization was observed in both healthy and malignant tissues. In addition, a marked decrease in Zn was found in the lateral lobe of the prostate obtained from mice with PCa. Conclusion: We demonstrate here that µ-SRXRF is a useful tool for monitoring the distribution of several elements including Zn and Gd in animal models of cancer. The optimized procedures for tissue preparation, processing, data collection, and analysis are described.
AB - Background: Contrast agents (CA) are administered in magnetic resonance imaging (MRI) clinical exams to measure tissue perfusion, enhance image contrast between adjacent tissues, or provide additional biochemical information in molecular MRI. The efficacy of a CA is determined by the tissue distribution of the agent and its concentration in the extracellular space of all tissues. Methods: In this work, micro-synchrotron radiation x-ray fluorescence (µ-SRXRF) was used to examine and characterize a gadolinium-based zinc-sensitive agent (GdL2) currently under development for detection of prostate cancer (PCa) by MRI. Prostate tissue samples were collected from control mice and mice with known PCa after an MRI exam that included injection of GdL2. The samples were raster scanned to investigate trends in Zn, Gd, Cu, Fe, S, P, and Ca. Results: Significant Zn and Gd co-localization was observed in both healthy and malignant tissues. In addition, a marked decrease in Zn was found in the lateral lobe of the prostate obtained from mice with PCa. Conclusion: We demonstrate here that µ-SRXRF is a useful tool for monitoring the distribution of several elements including Zn and Gd in animal models of cancer. The optimized procedures for tissue preparation, processing, data collection, and analysis are described.
KW - Contrast agent
KW - Gadolinium
KW - Prostate cancer
KW - Synchrotron radiation
KW - X-ray fluorescence
KW - Zinc
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U2 - 10.1016/j.jtemb.2022.127054
DO - 10.1016/j.jtemb.2022.127054
M3 - Article
C2 - 35939923
AN - SCOPUS:85135573353
SN - 0946-672X
VL - 74
JO - Journal of Trace Elements in Medicine and Biology
JF - Journal of Trace Elements in Medicine and Biology
M1 - 127054
ER -