TY - JOUR
T1 - Using arterial–venous analysis to characterize cancer metabolic consumption in patients
AU - Xiong, Nanxiang
AU - Gao, Xiaofei
AU - Zhao, Hongyang
AU - Cai, Feng
AU - Zhang, Fang cheng
AU - Yuan, Ye
AU - Liu, Weichao
AU - He, Fangping
AU - Zacharias, Lauren G.
AU - Lin, Hong
AU - Vu, Hieu S.
AU - Xing, Chao
AU - Yao, Dong Xiao
AU - Chen, Fei
AU - Luo, Benyan
AU - Sun, Wenzhi
AU - DeBerardinis, Ralph J.
AU - Xu, Hao
AU - Ge, Woo ping
N1 - Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Understanding tumor metabolism holds the promise of new insights into cancer biology, diagnosis and treatment. To assess human cancer metabolism, here we report a method to collect intra-operative samples of blood from an artery directly upstream and a vein directly downstream of a brain tumor, as well as samples from dorsal pedal veins of the same patients. After performing targeted metabolomic analysis, we characterize the metabolites consumed and produced by gliomas in vivo by comparing the arterial supply and venous drainage. N-acetylornithine, D-glucose, putrescine, and L-acetylcarnitine are consumed in relatively large amounts by gliomas. Conversely, L-glutamine, agmatine, and uridine 5-monophosphate are produced in relatively large amounts by gliomas. Further we verify that D-2-hydroxyglutarate (D-2HG) is high in venous plasma from patients with isocitrate dehydrogenases1 (IDH1) mutations. Through these paired comparisons, we can exclude the interpatient variation that is present in plasma samples usually taken from the cubital vein.
AB - Understanding tumor metabolism holds the promise of new insights into cancer biology, diagnosis and treatment. To assess human cancer metabolism, here we report a method to collect intra-operative samples of blood from an artery directly upstream and a vein directly downstream of a brain tumor, as well as samples from dorsal pedal veins of the same patients. After performing targeted metabolomic analysis, we characterize the metabolites consumed and produced by gliomas in vivo by comparing the arterial supply and venous drainage. N-acetylornithine, D-glucose, putrescine, and L-acetylcarnitine are consumed in relatively large amounts by gliomas. Conversely, L-glutamine, agmatine, and uridine 5-monophosphate are produced in relatively large amounts by gliomas. Further we verify that D-2-hydroxyglutarate (D-2HG) is high in venous plasma from patients with isocitrate dehydrogenases1 (IDH1) mutations. Through these paired comparisons, we can exclude the interpatient variation that is present in plasma samples usually taken from the cubital vein.
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U2 - 10.1038/s41467-020-16810-8
DO - 10.1038/s41467-020-16810-8
M3 - Article
C2 - 32576825
AN - SCOPUS:85086781413
SN - 2041-1723
VL - 11
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 3169
ER -