Use of latex particles for analysis of heterokaryon formation and cell fusion

Maura R. Levine; Rody P. Cox

doi:10.1007/BF01538870

Use of latex particles for analysis of heterokaryon formation and cell fusion

Maura R. Levine, Rody P. Cox

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Abstract

A simple method for the quick and accurate detection of cell fusion utilizing latex particles as cytoplasmic markers was developed and is reported here. The method is particularly useful for demonstrating human skin fibroblast heterokaryons. Ingestion of latex particles did not affect the growth of primary human and established BHK²¹/C₁₃ hamster fibroblasts. In addition, somatic cell hybridization between hypoxanthine phosphoribosyltransferase-deficient (HPRT^-) and thymidine kinase-deficient (TK^-) mutants of BHK²¹/C₁₃ was also unaffected by latex particle ingestion.

Original language	English (US)
Pages (from-to)	507-512
Number of pages	6
Journal	Somatic Cell Genetics
Volume	4
Issue number	4
DOIs	https://doi.org/10.1007/BF01538870
State	Published - Jul 1 1978

ASJC Scopus subject areas

Genetics

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title = "Use of latex particles for analysis of heterokaryon formation and cell fusion",

abstract = "A simple method for the quick and accurate detection of cell fusion utilizing latex particles as cytoplasmic markers was developed and is reported here. The method is particularly useful for demonstrating human skin fibroblast heterokaryons. Ingestion of latex particles did not affect the growth of primary human and established BHK21/C13 hamster fibroblasts. In addition, somatic cell hybridization between hypoxanthine phosphoribosyltransferase-deficient (HPRT-) and thymidine kinase-deficient (TK-) mutants of BHK21/C13 was also unaffected by latex particle ingestion.",

author = "Levine, {Maura R.} and Cox, {Rody P.}",

year = "1978",

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T1 - Use of latex particles for analysis of heterokaryon formation and cell fusion

AU - Levine, Maura R.

AU - Cox, Rody P.

PY - 1978/7/1

Y1 - 1978/7/1

N2 - A simple method for the quick and accurate detection of cell fusion utilizing latex particles as cytoplasmic markers was developed and is reported here. The method is particularly useful for demonstrating human skin fibroblast heterokaryons. Ingestion of latex particles did not affect the growth of primary human and established BHK21/C13 hamster fibroblasts. In addition, somatic cell hybridization between hypoxanthine phosphoribosyltransferase-deficient (HPRT-) and thymidine kinase-deficient (TK-) mutants of BHK21/C13 was also unaffected by latex particle ingestion.

AB - A simple method for the quick and accurate detection of cell fusion utilizing latex particles as cytoplasmic markers was developed and is reported here. The method is particularly useful for demonstrating human skin fibroblast heterokaryons. Ingestion of latex particles did not affect the growth of primary human and established BHK21/C13 hamster fibroblasts. In addition, somatic cell hybridization between hypoxanthine phosphoribosyltransferase-deficient (HPRT-) and thymidine kinase-deficient (TK-) mutants of BHK21/C13 was also unaffected by latex particle ingestion.

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DO - 10.1007/BF01538870

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JO - Somatic Cell Genetics

JF - Somatic Cell Genetics

IS - 4

ER -

Use of latex particles for analysis of heterokaryon formation and cell fusion

Abstract

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