Urine and fecal microbiota in a canine model of bladder cancer and comparison of canine and human urine microbiota

Ryan Mrofchak; Christopher Madden; Morgan V. Evans; William C. Kisseberth; Deepika Dhawan; Deborah W. Knapp; Vanessa L. Hale

doi:10.1080/26895293.2022.2154858

Urine and fecal microbiota in a canine model of bladder cancer and comparison of canine and human urine microbiota

Ryan Mrofchak, Christopher Madden, Morgan V. Evans, William C. Kisseberth, Deepika Dhawan, Deborah W. Knapp, Vanessa L. Hale

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Urothelial carcinoma (UC) is the tenth most diagnosed cancer in humans worldwide. Dogs are a robust model for invasive UC as tumor development and progression is similar in humans and dogs. Recent studies in humans have revealed alterations in urine microbiota in individuals with UC; however, microbial alterations in dogs with UC have not been evaluated. The objective of this pilot study was to compare the urine and fecal microbiota of dogs with UC and matched healthy controls. DNA was extracted from urine and fecal samples followed by 16S rRNA sequencing and analyses using QIIME2 and R. Dogs with UC had significantly decreased microbial diversity (Kruskal–Wallis; Shannon, p = 0.048) and altered microbial composition (PERMANOVA: Unweighted UniFrac, p = 0.011) in urine, but not fecal samples. The relative abundance of Fusobacterium was also increased, although not significantly, in urine and fecal samples of dogs with UC. A comparison of canine and human urine microbiota further revealed similarities in dominant microbial taxa across both host species. This study supports the value of dogs as a model for studies on bladder cancer and urine microbiota, and it provides a foundation for future work exploring host-microbe dynamics in UC carcinogenesis, prognosis, and treatment.

Original language	English (US)
Pages (from-to)	1245-1263
Number of pages	19
Journal	All Life
Volume	15
Issue number	1
DOIs	https://doi.org/10.1080/26895293.2022.2154858
State	Published - 2022
Externally published	Yes

Keywords

Bladder cancer
dogs
feces
gastrointestinal microbiome
pilot study
urine

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

Access to Document

10.1080/26895293.2022.2154858

Cite this

@article{1e0ece368a504128b335caa4ca61fb5a,

title = "Urine and fecal microbiota in a canine model of bladder cancer and comparison of canine and human urine microbiota",

abstract = "Urothelial carcinoma (UC) is the tenth most diagnosed cancer in humans worldwide. Dogs are a robust model for invasive UC as tumor development and progression is similar in humans and dogs. Recent studies in humans have revealed alterations in urine microbiota in individuals with UC; however, microbial alterations in dogs with UC have not been evaluated. The objective of this pilot study was to compare the urine and fecal microbiota of dogs with UC and matched healthy controls. DNA was extracted from urine and fecal samples followed by 16S rRNA sequencing and analyses using QIIME2 and R. Dogs with UC had significantly decreased microbial diversity (Kruskal–Wallis; Shannon, p = 0.048) and altered microbial composition (PERMANOVA: Unweighted UniFrac, p = 0.011) in urine, but not fecal samples. The relative abundance of Fusobacterium was also increased, although not significantly, in urine and fecal samples of dogs with UC. A comparison of canine and human urine microbiota further revealed similarities in dominant microbial taxa across both host species. This study supports the value of dogs as a model for studies on bladder cancer and urine microbiota, and it provides a foundation for future work exploring host-microbe dynamics in UC carcinogenesis, prognosis, and treatment.",

keywords = "Bladder cancer, dogs, feces, gastrointestinal microbiome, pilot study, urine",

author = "Ryan Mrofchak and Christopher Madden and Evans, {Morgan V.} and Kisseberth, {William C.} and Deepika Dhawan and Knapp, {Deborah W.} and Hale, {Vanessa L.}",

note = "Funding Information: for this project was provided by the Ohio State University Department of Veterinary Preventive Medicine (VLH, RM, CM), the Ohio State University Infectious Diseases Institute (VLH, RM, CM), the Ohio State University College of Veterinary Medicine Canine Funds, College of Veterinary Medicine, Ohio State University Funds (VLH, WK, DK), and the Ohio State University College of Public Health Collaborative Postdoctoral Research Award Program Award (MVE). We are grateful to all individuals involved in sample collection at Purdue University College of Veterinary Medicine (West Lafayette, IN, USA) and to the dogs and dog owners who participated in this study. We also acknowledge the Ohio Supercomputer Center (Columbus, Ohio, USA, established 1987) for computing resources used in this study. Publisher Copyright: {\textcopyright} 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",

year = "2022",

doi = "10.1080/26895293.2022.2154858",

language = "English (US)",

volume = "15",

pages = "1245--1263",

journal = "All Life",

issn = "2689-5293",

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TY - JOUR

T1 - Urine and fecal microbiota in a canine model of bladder cancer and comparison of canine and human urine microbiota

AU - Mrofchak, Ryan

AU - Madden, Christopher

AU - Evans, Morgan V.

AU - Kisseberth, William C.

AU - Dhawan, Deepika

AU - Knapp, Deborah W.

AU - Hale, Vanessa L.

N1 - Funding Information: for this project was provided by the Ohio State University Department of Veterinary Preventive Medicine (VLH, RM, CM), the Ohio State University Infectious Diseases Institute (VLH, RM, CM), the Ohio State University College of Veterinary Medicine Canine Funds, College of Veterinary Medicine, Ohio State University Funds (VLH, WK, DK), and the Ohio State University College of Public Health Collaborative Postdoctoral Research Award Program Award (MVE). We are grateful to all individuals involved in sample collection at Purdue University College of Veterinary Medicine (West Lafayette, IN, USA) and to the dogs and dog owners who participated in this study. We also acknowledge the Ohio Supercomputer Center (Columbus, Ohio, USA, established 1987) for computing resources used in this study. Publisher Copyright: © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

PY - 2022

Y1 - 2022

N2 - Urothelial carcinoma (UC) is the tenth most diagnosed cancer in humans worldwide. Dogs are a robust model for invasive UC as tumor development and progression is similar in humans and dogs. Recent studies in humans have revealed alterations in urine microbiota in individuals with UC; however, microbial alterations in dogs with UC have not been evaluated. The objective of this pilot study was to compare the urine and fecal microbiota of dogs with UC and matched healthy controls. DNA was extracted from urine and fecal samples followed by 16S rRNA sequencing and analyses using QIIME2 and R. Dogs with UC had significantly decreased microbial diversity (Kruskal–Wallis; Shannon, p = 0.048) and altered microbial composition (PERMANOVA: Unweighted UniFrac, p = 0.011) in urine, but not fecal samples. The relative abundance of Fusobacterium was also increased, although not significantly, in urine and fecal samples of dogs with UC. A comparison of canine and human urine microbiota further revealed similarities in dominant microbial taxa across both host species. This study supports the value of dogs as a model for studies on bladder cancer and urine microbiota, and it provides a foundation for future work exploring host-microbe dynamics in UC carcinogenesis, prognosis, and treatment.

AB - Urothelial carcinoma (UC) is the tenth most diagnosed cancer in humans worldwide. Dogs are a robust model for invasive UC as tumor development and progression is similar in humans and dogs. Recent studies in humans have revealed alterations in urine microbiota in individuals with UC; however, microbial alterations in dogs with UC have not been evaluated. The objective of this pilot study was to compare the urine and fecal microbiota of dogs with UC and matched healthy controls. DNA was extracted from urine and fecal samples followed by 16S rRNA sequencing and analyses using QIIME2 and R. Dogs with UC had significantly decreased microbial diversity (Kruskal–Wallis; Shannon, p = 0.048) and altered microbial composition (PERMANOVA: Unweighted UniFrac, p = 0.011) in urine, but not fecal samples. The relative abundance of Fusobacterium was also increased, although not significantly, in urine and fecal samples of dogs with UC. A comparison of canine and human urine microbiota further revealed similarities in dominant microbial taxa across both host species. This study supports the value of dogs as a model for studies on bladder cancer and urine microbiota, and it provides a foundation for future work exploring host-microbe dynamics in UC carcinogenesis, prognosis, and treatment.

KW - Bladder cancer

KW - dogs

KW - feces

KW - gastrointestinal microbiome

KW - pilot study

KW - urine

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SN - 2689-5293

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JO - All Life

JF - All Life

IS - 1

ER -

Urine and fecal microbiota in a canine model of bladder cancer and comparison of canine and human urine microbiota

Abstract

Keywords

ASJC Scopus subject areas

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