TY - JOUR
T1 - Upstream regulatory regions required to stabilize binding to the TATA sequence in an adesovirus early promoter
AU - Garcia, Joseph
AU - Wu, Food
AU - Gaynor, Richard
N1 - Funding Information:
ACKNOWLEDGEMENTS We would like to thank Am1 e 8erk for suggestions 1n preparing this manuscript, Danny Hong and Janna Chao for technical assistance, and Wendy Aft for preparation of this manuscript. This work was supported by a grant CA 30981 from the NIH, and JFRA-146 from the American Cancer Society. J.G. was supported by NIH grant 6H0-80942 1n the UCLA Medical Scientist Training Program.
PY - 1987/10/26
Y1 - 1987/10/26
N2 - Of the five early adenovirus promoters, the early region 3 (E3) promoter is one of the most strongly induced by the E1A protein. To identify cellular proteins involved in both the basa1 and ElA-induced transcriptional regulation of the E3 promoter, ONase I footprinting using partially purified Hela cell extracts was performed. Four regions of the E3 promoter serve as binding domains for cellular proteins. These regions are found between -156 to -179 (site IV), -83 to -103 (site III), -47 to -67 (site II), and -16 to -37 (site I), relative to the start of transcription. Examination of the DNA sequences in each binding domain suggests that site III likely serves as a binding site for activator protein 1 (AP-1), site II for the cyclic AMP regulatory element binding protein (CREB), and site I for a TATA binding factor. The factors binding to either site II or III were sufficient to stabilize binding to the TATA sequence (site I). Mutagenesis studies Indicated that both sites II and III, in addition to site I, are needed for complete basal and ElA-induced transcription. These results suggest that multiple cellular factors are Involved in both the basal and EIA-induced transcriptional regulation of the E3 promoter, and that either of two upstream regions are capable of stabilizing factor binding to the TATA sequence.
AB - Of the five early adenovirus promoters, the early region 3 (E3) promoter is one of the most strongly induced by the E1A protein. To identify cellular proteins involved in both the basa1 and ElA-induced transcriptional regulation of the E3 promoter, ONase I footprinting using partially purified Hela cell extracts was performed. Four regions of the E3 promoter serve as binding domains for cellular proteins. These regions are found between -156 to -179 (site IV), -83 to -103 (site III), -47 to -67 (site II), and -16 to -37 (site I), relative to the start of transcription. Examination of the DNA sequences in each binding domain suggests that site III likely serves as a binding site for activator protein 1 (AP-1), site II for the cyclic AMP regulatory element binding protein (CREB), and site I for a TATA binding factor. The factors binding to either site II or III were sufficient to stabilize binding to the TATA sequence (site I). Mutagenesis studies Indicated that both sites II and III, in addition to site I, are needed for complete basal and ElA-induced transcription. These results suggest that multiple cellular factors are Involved in both the basal and EIA-induced transcriptional regulation of the E3 promoter, and that either of two upstream regions are capable of stabilizing factor binding to the TATA sequence.
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U2 - 10.1093/nar/15.20.8367
DO - 10.1093/nar/15.20.8367
M3 - Article
C2 - 2959908
AN - SCOPUS:0023665941
SN - 0305-1048
VL - 15
SP - 8367
EP - 8385
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 20
ER -