TY - JOUR
T1 - Upregulation of Krüppel-like factor 6 in the mouse hippocampus after pilocarpine-induced status epilepticus
AU - Jeong, K. H.
AU - Lee, K. E.
AU - Kim, S. Y.
AU - Cho, K. O.
N1 - Funding Information:
This work was supported by the Korea Science and Engineering Foundation (R13-2002-005-04002-0) through the MRC for Cell Death Disease Research Center at The Catholic University of Korea and by the Biogreen21 Program (20100301-061-131-001-03-00), Rural Development Administration, Korea.
PY - 2011/7/14
Y1 - 2011/7/14
N2 - Krüppel-like factor 6 (KLF6) is a transcriptional regulator involved in a broad range of cellular processes. To date, however, the expression of KLF6 in brains with pathophysiological conditions, such as epilepsy, has not been reported. Therefore, the present study investigated the temporal pattern of KLF6 expression in the mouse hippocampus and identified cell types expressing KLF6 after pilocarpine-induced status epilepticus (SE). Seizures were induced by administrating pilocarpine hydrochloride (280 mg/kg, i.p.) 30 min after an injection of atropine methyl nitrate (3 mg/kg, i.p.). Pilocarpine- and saline-injected animals were sacrificed 1, 3, 7, 14, or 28 days after the onset of SE. Immunohistochemistry showed that the proportion of KLF6-positive cells increased in the hippocampus 1 day after SE onset, peaked at 3 days after SE, and then gradually decreased until 28 days after SE, consistent with the results from our immunoblot analysis. Cells expressing increased levels of KLF6 following pilocarpine-induced SE also expressed GFAP and Ox-42, markers for astrocytes and microglia, respectively. Quantitative analysis revealed that astrocytes were the major type of KLF6-expressing glial cells. These cells also expressed heat shock protein 47 (HSP47), a collagen-specific molecular chaperone. This is the first report showing that KLF6 is inducible in the hippocampus and may be associated with glial responses, especially HSP47-related tissue remodeling after pilocarpine-induced SE.
AB - Krüppel-like factor 6 (KLF6) is a transcriptional regulator involved in a broad range of cellular processes. To date, however, the expression of KLF6 in brains with pathophysiological conditions, such as epilepsy, has not been reported. Therefore, the present study investigated the temporal pattern of KLF6 expression in the mouse hippocampus and identified cell types expressing KLF6 after pilocarpine-induced status epilepticus (SE). Seizures were induced by administrating pilocarpine hydrochloride (280 mg/kg, i.p.) 30 min after an injection of atropine methyl nitrate (3 mg/kg, i.p.). Pilocarpine- and saline-injected animals were sacrificed 1, 3, 7, 14, or 28 days after the onset of SE. Immunohistochemistry showed that the proportion of KLF6-positive cells increased in the hippocampus 1 day after SE onset, peaked at 3 days after SE, and then gradually decreased until 28 days after SE, consistent with the results from our immunoblot analysis. Cells expressing increased levels of KLF6 following pilocarpine-induced SE also expressed GFAP and Ox-42, markers for astrocytes and microglia, respectively. Quantitative analysis revealed that astrocytes were the major type of KLF6-expressing glial cells. These cells also expressed heat shock protein 47 (HSP47), a collagen-specific molecular chaperone. This is the first report showing that KLF6 is inducible in the hippocampus and may be associated with glial responses, especially HSP47-related tissue remodeling after pilocarpine-induced SE.
KW - Heat shock protein 47
KW - Hippocampus
KW - Krüppel-like factor 6
KW - Mice
KW - Status epilepticus
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U2 - 10.1016/j.neuroscience.2011.02.046
DO - 10.1016/j.neuroscience.2011.02.046
M3 - Article
C2 - 21362463
AN - SCOPUS:79957978263
SN - 0306-4522
VL - 186
SP - 170
EP - 178
JO - Neuroscience
JF - Neuroscience
ER -