Upregulation of Escherichia coli heat-stable enterotoxin receptor in regenerating rat liver

D. W. Laney, J. A. Bezerra, J. L. Kosiba, S. J.F. Degen, M. B. Cohen

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Guanylate cyclase C (GC-C) is a transmembrane protein that serves as a receptor for the recently characterized endogenous ligand guanylin and for Escherichia coli heat-stable toxin (ST(a)). Binding of either guanylin or ST(a) to intestinal GC-C results in net chloride secretion. Although GC-C is expressed in the rat intestine throughout life, its expression in the rat liver has previously been shown to occur only during the perinatal period. As a step toward elucidating the role of this receptor in the liver, we tested the hypothesis that GC-C mRNA expression could be induced in the adult rat liver following 1) partial hepatectomy, a stimulus for hepatocyte proliferation; 2) intraperitoneal carbon tetrachloride injection, a model of hepatocyte regeneration in the presence of inflammatory changes; and 3) subcutaneous turpentine injection, which generates an acute phase response without hepatocyte proliferation. We demonstrated expression of GC-C mRNA in the regenerating rat liver following, either partial hepatectomy or CCl4- induced hepatic necrosis. We have also shown that GC-C mRNA expression occurred in association with an acute phase reaction. Coordinate with the expression of GC-C mRNA, there was upregulation of radiolabeled ST(a) binding to liver plasma membranes prepared from turpentine-treated rats. Maximal expression of GC-C occurred in preparations enriched for the canalicular domain. Although the function of GC-C in the liver is unknown, ocalization to the canalicular domain would be consistent with a role for GC-C in hepatic chloride secretion, especially in the perinatal liver and during hepatocyte regeneration.

Original languageEnglish (US)
Pages (from-to)G899-G906
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number5 29-5
StatePublished - 1994
Externally publishedYes


  • acute phase response
  • carbon tetrachloride
  • chloride secretion
  • guanylate cyclase C
  • guanylin
  • partial hepatectomy
  • turpentine

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)


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