Universal light-sheet generation with field synthesis

Bo Jui Chang; Mark Kittisopikul; Kevin M. Dean; Philippe Roudot; Erik S. Welf; Reto Fiolka

doi:10.1038/s41592-019-0327-9

Universal light-sheet generation with field synthesis

Bo Jui Chang, Mark Kittisopikul, Kevin M. Dean, Philippe Roudot, Erik S. Welf, Reto Fiolka

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral beam scanning.

Original language	English (US)
Pages (from-to)	235-238
Number of pages	4
Journal	Nature methods
Volume	16
Issue number	3
DOIs	https://doi.org/10.1038/s41592-019-0327-9
State	Published - Mar 1 2019

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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AU - Welf, Erik S.

AU - Fiolka, Reto

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Universal light-sheet generation with field synthesis

Abstract

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