TY - JOUR
T1 - UBL1, a human ubiquitin-like protein associating with human RAD51/RAD52 proteins
AU - Shen, Zhiyuan
AU - Pardington-Purtymun, Paige E.
AU - Comeaux, Jarmon C.
AU - Moyzis, Robert K.
AU - Chen, David J.
N1 - Funding Information:
This research was supported by the U.S. Department of Energy and NIH Grant CA50519 to D.J.C. and by a Director's postdoctoral fellowship from Los Alamos National Laboratory to Z.S. We thank Drs. Morton E. Bradbury, Scott Peterson, and Robert Cary for helpful discussions and critical reading of the manuscript.
PY - 1996/9/1
Y1 - 1996/9/1
N2 - The RAD51/RAD52-dependent DNA repair pathway is involved in DNA recombination and DNA double-strand break repair in yeast. Although many proteins in the RAD51/RAD52-dependent DNA repair pathway have been identified in yeast, a novel protein(s) that functions with RAD51/RAD52 may also exist in humans. Using a yeast two-hybrid system, we have identified a 12-kDa protein that associates with the human RAD51 and RAD52 proteins. This protein shares significant amino acid homology with the yeast protein SMT3, which functionally associates with the yeast mitosis fidelity protein MIF2. It also shares moderate homology with ubiquitin and several other proteins, including the N-terminus of the RAD23 protein and a ubiquitin cross reacting protein. Therefore, the gene is tentatively designated UBL1 for ubiquitin-like 1. The UBL1 mRNA is expressed in many human tissues, most highly in testis. The UBL1 gene is mapped to chromosome 2q32.2-q33, and a related sequence may be located on chromosome 1q23-q25.
AB - The RAD51/RAD52-dependent DNA repair pathway is involved in DNA recombination and DNA double-strand break repair in yeast. Although many proteins in the RAD51/RAD52-dependent DNA repair pathway have been identified in yeast, a novel protein(s) that functions with RAD51/RAD52 may also exist in humans. Using a yeast two-hybrid system, we have identified a 12-kDa protein that associates with the human RAD51 and RAD52 proteins. This protein shares significant amino acid homology with the yeast protein SMT3, which functionally associates with the yeast mitosis fidelity protein MIF2. It also shares moderate homology with ubiquitin and several other proteins, including the N-terminus of the RAD23 protein and a ubiquitin cross reacting protein. Therefore, the gene is tentatively designated UBL1 for ubiquitin-like 1. The UBL1 mRNA is expressed in many human tissues, most highly in testis. The UBL1 gene is mapped to chromosome 2q32.2-q33, and a related sequence may be located on chromosome 1q23-q25.
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U2 - 10.1006/geno.1996.0462
DO - 10.1006/geno.1996.0462
M3 - Article
C2 - 8812453
AN - SCOPUS:0030249870
SN - 0888-7543
VL - 36
SP - 271
EP - 279
JO - Genomics
JF - Genomics
IS - 2
ER -