Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase

Yong Gang Zheng; Yong Li; Jian Feng Chen; Wei Hong Jiang; Guo Ping Zhao; En Duo Wang

doi:10.1023/A:1012452516082

Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase

Yong Gang Zheng, Yong Li, Jian Feng Chen, Wei Hong Jiang, Guo Ping Zhao, En Duo Wang

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Two novel engineered bacteria, BL21(DE3)/pETCA1S and TG1/pSuperCA1S, were obtained which can secretory express the gene encoding glutaryl 7-amino-cephalosporanic acid acylase (GL-7ACA acylase) from Pseudomonas sp. 130 with high activity. The growth conditions of transformants for overproduction of GL-7ACA acylase were optimized: in intact cells of BL21(DE3)/pETCA1S and TG1/pSuperCA1S the activity of GL-7ACA acylase was 415 and 600 units g^-1 dry cells, respectively. The highest specific activity of GL-7-ACA acylase is in the intact cell as compared with that of transformants constructed in our laboratory. In fiftieth generation of mutants transferred on agar plates the specific activity of GL-7ACA acylase remained constant.

Original language	English (US)
Pages (from-to)	1781-1787
Number of pages	7
Journal	Biotechnology Letters
Volume	23
Issue number	21
DOIs	https://doi.org/10.1023/A:1012452516082
State	Published - 2001
Externally published	Yes

Keywords

Cloramphenicol
Glutaryl 7-amino-cephalosporanic acid acylase
Kanamycin
Secretory expression

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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@article{f68e0d7dbdbe40ed81ef8417e7309508,

title = "Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase",

abstract = "Two novel engineered bacteria, BL21(DE3)/pETCA1S and TG1/pSuperCA1S, were obtained which can secretory express the gene encoding glutaryl 7-amino-cephalosporanic acid acylase (GL-7ACA acylase) from Pseudomonas sp. 130 with high activity. The growth conditions of transformants for overproduction of GL-7ACA acylase were optimized: in intact cells of BL21(DE3)/pETCA1S and TG1/pSuperCA1S the activity of GL-7ACA acylase was 415 and 600 units g-1 dry cells, respectively. The highest specific activity of GL-7-ACA acylase is in the intact cell as compared with that of transformants constructed in our laboratory. In fiftieth generation of mutants transferred on agar plates the specific activity of GL-7ACA acylase remained constant.",

keywords = "Cloramphenicol, Glutaryl 7-amino-cephalosporanic acid acylase, Kanamycin, Secretory expression",

author = "Zheng, {Yong Gang} and Yong Li and Chen, {Jian Feng} and Jiang, {Wei Hong} and Zhao, {Guo Ping} and Wang, {En Duo}",

note = "Funding Information: This work was funded by the 863 project of China (Grant no. 103-13-02-01).",

year = "2001",

doi = "10.1023/A:1012452516082",

language = "English (US)",

volume = "23",

pages = "1781--1787",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "21",

}

TY - JOUR

T1 - Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase

AU - Zheng, Yong Gang

AU - Li, Yong

AU - Chen, Jian Feng

AU - Jiang, Wei Hong

AU - Zhao, Guo Ping

AU - Wang, En Duo

N1 - Funding Information: This work was funded by the 863 project of China (Grant no. 103-13-02-01).

PY - 2001

Y1 - 2001

N2 - Two novel engineered bacteria, BL21(DE3)/pETCA1S and TG1/pSuperCA1S, were obtained which can secretory express the gene encoding glutaryl 7-amino-cephalosporanic acid acylase (GL-7ACA acylase) from Pseudomonas sp. 130 with high activity. The growth conditions of transformants for overproduction of GL-7ACA acylase were optimized: in intact cells of BL21(DE3)/pETCA1S and TG1/pSuperCA1S the activity of GL-7ACA acylase was 415 and 600 units g-1 dry cells, respectively. The highest specific activity of GL-7-ACA acylase is in the intact cell as compared with that of transformants constructed in our laboratory. In fiftieth generation of mutants transferred on agar plates the specific activity of GL-7ACA acylase remained constant.

AB - Two novel engineered bacteria, BL21(DE3)/pETCA1S and TG1/pSuperCA1S, were obtained which can secretory express the gene encoding glutaryl 7-amino-cephalosporanic acid acylase (GL-7ACA acylase) from Pseudomonas sp. 130 with high activity. The growth conditions of transformants for overproduction of GL-7ACA acylase were optimized: in intact cells of BL21(DE3)/pETCA1S and TG1/pSuperCA1S the activity of GL-7ACA acylase was 415 and 600 units g-1 dry cells, respectively. The highest specific activity of GL-7-ACA acylase is in the intact cell as compared with that of transformants constructed in our laboratory. In fiftieth generation of mutants transferred on agar plates the specific activity of GL-7ACA acylase remained constant.

KW - Cloramphenicol

KW - Glutaryl 7-amino-cephalosporanic acid acylase

KW - Kanamycin

KW - Secretory expression

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UR - http://www.scopus.com/inward/citedby.url?scp=0035181565&partnerID=8YFLogxK

U2 - 10.1023/A:1012452516082

DO - 10.1023/A:1012452516082

M3 - Article

AN - SCOPUS:0035181565

SN - 0141-5492

VL - 23

SP - 1781

EP - 1787

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 21

ER -

Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase

Abstract

Keywords

ASJC Scopus subject areas

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