Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells

Lance S. Terada; Irene R. Willingham; David M. Guidot; Gayle N. Shibao; Glenn W. Kindt; John E. Repine

doi:10.1007/BF00917511

Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells

Lance S. Terada, Irene R. Willingham, David M. Guidot, Gayle N. Shibao, Glenn W. Kindt, John E. Repine

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22 Scopus citations

Abstract

Exposure to recombinant human tumor necrosis factor-alpha (TNF-α) or calcium ionophore (A23187) for 4 h increased (P< 0.05) lactate dehydrogenase (LDH) release from cultured bovine brain endothelial cells (EC). In contrast, treatment with endotoxin or interleukin-1 did not increase (P> 0.05) LDH release from brain EC. Pretreatment with tungsten decreased (P< 0.05) xanthine oxidase activity in brain EC and decreased (P< 0.05) LDH release from brain EC following exposure to TNF. Our results suggest that TNF-α injures brain microvascular EC and that this effect may be mediated by xanthine oxidase.

Original language	English (US)
Pages (from-to)	13-19
Number of pages	7
Journal	Inflammation
Volume	16
Issue number	1
DOIs	https://doi.org/10.1007/BF00917511
State	Published - Feb 1992

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells",

abstract = "Exposure to recombinant human tumor necrosis factor-alpha (TNF-α) or calcium ionophore (A23187) for 4 h increased (P< 0.05) lactate dehydrogenase (LDH) release from cultured bovine brain endothelial cells (EC). In contrast, treatment with endotoxin or interleukin-1 did not increase (P> 0.05) LDH release from brain EC. Pretreatment with tungsten decreased (P< 0.05) xanthine oxidase activity in brain EC and decreased (P< 0.05) LDH release from brain EC following exposure to TNF. Our results suggest that TNF-α injures brain microvascular EC and that this effect may be mediated by xanthine oxidase.",

author = "Terada, {Lance S.} and Willingham, {Irene R.} and Guidot, {David M.} and Shibao, {Gayle N.} and Kindt, {Glenn W.} and Repine, {John E.}",

year = "1992",

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doi = "10.1007/BF00917511",

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T1 - Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells

AU - Terada, Lance S.

AU - Willingham, Irene R.

AU - Guidot, David M.

AU - Shibao, Gayle N.

AU - Kindt, Glenn W.

AU - Repine, John E.

PY - 1992/2

Y1 - 1992/2

N2 - Exposure to recombinant human tumor necrosis factor-alpha (TNF-α) or calcium ionophore (A23187) for 4 h increased (P< 0.05) lactate dehydrogenase (LDH) release from cultured bovine brain endothelial cells (EC). In contrast, treatment with endotoxin or interleukin-1 did not increase (P> 0.05) LDH release from brain EC. Pretreatment with tungsten decreased (P< 0.05) xanthine oxidase activity in brain EC and decreased (P< 0.05) LDH release from brain EC following exposure to TNF. Our results suggest that TNF-α injures brain microvascular EC and that this effect may be mediated by xanthine oxidase.

AB - Exposure to recombinant human tumor necrosis factor-alpha (TNF-α) or calcium ionophore (A23187) for 4 h increased (P< 0.05) lactate dehydrogenase (LDH) release from cultured bovine brain endothelial cells (EC). In contrast, treatment with endotoxin or interleukin-1 did not increase (P> 0.05) LDH release from brain EC. Pretreatment with tungsten decreased (P< 0.05) xanthine oxidase activity in brain EC and decreased (P< 0.05) LDH release from brain EC following exposure to TNF. Our results suggest that TNF-α injures brain microvascular EC and that this effect may be mediated by xanthine oxidase.

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Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells

Abstract

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