@article{2f13f93fe5de4b7d88a1ca2d918e03f5,
title = "Tumorigenic Cells Are Common in Mouse MPNSTs but Their Frequency Depends upon Tumor Genotype and Assay Conditions",
abstract = "Tumor-initiating cells have been suggested to be rare in many cancers. We tested this in mouse malignant peripheral nerve sheath tumors (MPNSTs) and found that 18% of primary and 49% of passaged MPNST cells from Nf1+/-; Ink4a/Arf-/- mice formed tumors upon transplantation, whereas only 1.8% to 2.6% of MPNST cells from Nf1+/-; p53+/- mice did. MPNST cells of both genotypes require laminin binding to β1-integrin for clonogenic growth. Most MPNST cells from Nf1+/-; Ink4a/Arf-/- mice expressed laminin, whereas most MPNST cells from Nf1+/-; p53+/- mice did not. Exogenous laminin increased the percentage of MPNST cells from Nf1+/-; p53+/- but not Nf1+/-; Ink4a/Arf-/- mice that formed tumorigenic colonies. Tumor-forming potential is common among MPNST cells, but the assay conditions required to detect it vary with tumor genotype.",
author = "Johanna Buchstaller and McKeever, {Paul E.} and Morrison, {Sean J.}",
note = "Funding Information: This work was supported by the National Institute of Neurological Disorder and Stroke (grant NS-040750), the Howard Hughes Medical Institute, and the Liddy Shriver Sarcoma Initiative. J.B. was supported by postdoctoral fellowships from the Swiss National Foundation of Science and the Children's Tumor Foundation. Thanks to Kristina Harter for technical assistance. Thanks to David Adams and Martin White for assistance with flow cytometry, which was partially supported by the University of Michigan (UM) Comprehensive Cancer Center (National Institutes of Health grant CA46592). Thanks to Maria Ripberger, Alan Burgess, and Nancy McAnsh for paraffin embedding, sectioning, and immunohistochemistry. Thanks to Amanda Welton in the UM Center for Molecular Imaging for magnetic resonance imaging. Thanks to Tyler Jacks for providing Nf1 germline knockout mice, Tyler Jacks and Karlyne Reilly for providing Nf1 +/− ; p53 +/− mice, and Ron DePinho for providing Ink4a/Arf mutant mice. ",
year = "2012",
month = feb,
day = "14",
doi = "10.1016/j.ccr.2011.12.027",
language = "English (US)",
volume = "21",
pages = "240--252",
journal = "Cancer Cell",
issn = "1535-6108",
publisher = "Cell Press",
number = "2",
}