Tryptophan as the fingerprint for distinguishing aggressiveness among breast cancer cell lines using native fluorescence spectroscopy

Lin Zhang; Yang Pu; Jianpeng Xue; Sebastião Pratavieira; Baogang Xu; Samuel Achilefu; Robert R. Alfano

doi:10.1117/1.JBO.19.3.037005

Tryptophan as the fingerprint for distinguishing aggressiveness among breast cancer cell lines using native fluorescence spectroscopy

Lin Zhang, Yang Pu, Jianpeng Xue, Sebastião Pratavieira, Baogang Xu, Samuel Achilefu, Robert R. Alfano

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Tryptophan is investigated as the key native marker in cells to determine the level of metastasis competence in breast cell lines using native fluorescence spectroscopy. The ratio of fluorescence intensity at 340 nm to intensity at 460 nm is associated with aggressiveness of the cancer cells. We found that the fluorescence of aggressive breast cancer cell has a much higher contribution from tryptophan compared with that from the normal cells and nonaggressive breast cancer cell.

Original language	English (US)
Article number	037005
Journal	Journal of biomedical optics
Volume	19
Issue number	3
DOIs	https://doi.org/10.1117/1.JBO.19.3.037005
State	Published - Mar 2014
Externally published	Yes

Keywords

aggressive breast cancer cells
cancer markers
metastasis
native fluorescence spectroscopy
tryptophan

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

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10.1117/1.JBO.19.3.037005

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title = "Tryptophan as the fingerprint for distinguishing aggressiveness among breast cancer cell lines using native fluorescence spectroscopy",

abstract = "Tryptophan is investigated as the key native marker in cells to determine the level of metastasis competence in breast cell lines using native fluorescence spectroscopy. The ratio of fluorescence intensity at 340 nm to intensity at 460 nm is associated with aggressiveness of the cancer cells. We found that the fluorescence of aggressive breast cancer cell has a much higher contribution from tryptophan compared with that from the normal cells and nonaggressive breast cancer cell.",

keywords = "aggressive breast cancer cells, cancer markers, metastasis, native fluorescence spectroscopy, tryptophan",

author = "Lin Zhang and Yang Pu and Jianpeng Xue and Sebasti{\~a}o Pratavieira and Baogang Xu and Samuel Achilefu and Alfano, {Robert R.}",

note = "Funding Information: This research is supported in part by U. S. Army Medical Research and Material Command grants numbered W81XWH-11-1-0335 and W81XWH-08-1-0717. Authors acknowledge Ms. Laura Sordillo in Department of Physics at the City College of the City University of New York for taking part in measuring NFL of MB-MDA-231. R.R.A. thanks Professor Bingmei Fu for use of her cell laboratory facility.",

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doi = "10.1117/1.JBO.19.3.037005",

language = "English (US)",

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T1 - Tryptophan as the fingerprint for distinguishing aggressiveness among breast cancer cell lines using native fluorescence spectroscopy

AU - Zhang, Lin

AU - Pu, Yang

AU - Xue, Jianpeng

AU - Pratavieira, Sebastião

AU - Xu, Baogang

AU - Achilefu, Samuel

AU - Alfano, Robert R.

N1 - Funding Information: This research is supported in part by U. S. Army Medical Research and Material Command grants numbered W81XWH-11-1-0335 and W81XWH-08-1-0717. Authors acknowledge Ms. Laura Sordillo in Department of Physics at the City College of the City University of New York for taking part in measuring NFL of MB-MDA-231. R.R.A. thanks Professor Bingmei Fu for use of her cell laboratory facility.

PY - 2014/3

Y1 - 2014/3

N2 - Tryptophan is investigated as the key native marker in cells to determine the level of metastasis competence in breast cell lines using native fluorescence spectroscopy. The ratio of fluorescence intensity at 340 nm to intensity at 460 nm is associated with aggressiveness of the cancer cells. We found that the fluorescence of aggressive breast cancer cell has a much higher contribution from tryptophan compared with that from the normal cells and nonaggressive breast cancer cell.

AB - Tryptophan is investigated as the key native marker in cells to determine the level of metastasis competence in breast cell lines using native fluorescence spectroscopy. The ratio of fluorescence intensity at 340 nm to intensity at 460 nm is associated with aggressiveness of the cancer cells. We found that the fluorescence of aggressive breast cancer cell has a much higher contribution from tryptophan compared with that from the normal cells and nonaggressive breast cancer cell.

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KW - cancer markers

KW - metastasis

KW - native fluorescence spectroscopy

KW - tryptophan

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Tryptophan as the fingerprint for distinguishing aggressiveness among breast cancer cell lines using native fluorescence spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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