Abstract
We have shown that elevated plasma d-glucose levels in experimentally-induced diabetic nude athymic rats can be reduced by intraperitoneal transplantation of microcarrier-attached insulin producing β cells from the mouse pancreatic β cell line, β TC-1. The reduction in the level of hyperglycemia was observed as early as two days following cell transplantation and was associated with a concomitant increase in plasma insulin levels. β TC-1 cell transplanted diabetic rats had plasma d-glucose levels similar to those found in non-diabetic control animals and remained normoglycemic throughout the 39 day experimental period. The β TC-1 cell transplanted diabetic rats also had near normalization of body weight, food and water intake and of urine output when compared to control diabetic and non-diabetic rats. Similarly, they exhibited improved blood glucose clearance following intravenous d-glucose administration. These results suggest that β TC-1 cells regulate d-glucose homeostasis following transplantation into diabetic rat recipients in a manner similar to that of endogenous pancreatic β cells.
Original language | English (US) |
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Pages (from-to) | 157-164 |
Number of pages | 8 |
Journal | Diabetes Research and Clinical Practice |
Volume | 14 |
Issue number | 3 |
DOIs | |
State | Published - Dec 1991 |
Keywords
- Cell therapy
- Insulin-dependent diabetes mellitus
- Rat
- Streptozotocin induced diabetes
- β cell transplantation
ASJC Scopus subject areas
- Internal Medicine
- Endocrinology, Diabetes and Metabolism
- Endocrinology