Transmembrane IV of the high-affinity sodium-glucose cotransporter participates in sugar binding

Tiemin Liu, Bryan Lo, Pam Speight, Mel Silverman

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Investigation of the structure/function relationships of the sodium-glucose transporter (SGLT1) is crucial to understanding the cotransporter mechanism. In the present study, we used cysteine-scanning mutagenesis and chemical modification by methanethiosulfonate (MTS) derivatives to test whether predicted transmembrane IV participates in sugar binding. Five charged and polar residues (K139, Q142, T156, K157, and D161) and two glucose/galactose malabsorption missense mutations (I147 and S159) were replaced with cysteine. Mutants I147C, T156C, and K157C exhibited sufficient expression to be studied in detail using the two-electrode voltage-clamp method in Xenopus laevis oocytes and COS-7 cells. I147C was similar in function to wild-type and was not studied further. Mutation of lysine-157 to cysteine (K157C) causes loss of phloridzin and α-methyl-D-glucopyranoside (αMG) binding. These functions are restored by chemical modification with positively charged (2-aminoethyl) methanethiosulfonate hydrobromide (MTSEA). Mutation of threonine-156 to cysteine (T156C) reduces the affinity of αMG and phloridzin for T156C by ∼5-fold and ∼20-fold, respectively. In addition, phloridzin protects cysteine-156 in T156C from alkylation by MTSEA. Therefore, the presence of a positive charge or a polar residue at 157 and 156, respectively, affects sugar binding and sugar-induced Na+ currents.

Original languageEnglish (US)
Pages (from-to)C64-C72
JournalAmerican Journal of Physiology - Cell Physiology
Issue number1
StatePublished - Jul 2008


  • Chemical modification by methanethiosulfonate reagents
  • Cysteine scanning mutagenesis

ASJC Scopus subject areas

  • Physiology
  • Cell Biology


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