Abstract
Gene expression by herpes simplex virus type 1 (HSV-1) results in the synthesis of three temporal classes of viral proteins. The three classes of viral proteins are expressed in a cascade manner of sequential dependency. The molecular mechanisms that account for the HSV-1 protein synthesis cascade are poorly understood. In order to provide a detailed description of the metabolic levels at which HSV-1 protein synthesis is regulated, we have measured transcription rates and mRNA accumulation levels for 11 HSV-1 genes. These measurements were made over a time-course of infection in the presence or absence of metabolic inhibitors of either viral protein synthesis or viral DNA synthesis. Our observations show that the protein synthesis cascade of HSV-1 is established as a consequence of mechanisms that regulate both the transcription and accumulation of viral messenger RNA.
Original language | English (US) |
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Pages (from-to) | 819-833 |
Number of pages | 15 |
Journal | Journal of Molecular Biology |
Volume | 195 |
Issue number | 4 |
DOIs | |
State | Published - Jun 20 1987 |
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology