TY - JOUR
T1 - Transcriptional analysis of mouse skeletal myofiber diversity and adaptation to endurance exercise
AU - Wu, Hai
AU - Gallardo, Teresa
AU - Olson, Eric N.
AU - Williams, R. Sanders
AU - Shohet, Ralph V.
N1 - Funding Information:
This work was supported by grants from the NHLBI Program in Genome Applications (RVS), and the NIH (RVS, RSW and ENO).
PY - 2003
Y1 - 2003
N2 - Vertebrate skeletal myofibers are heterogeneous with respect to their metabolic, contractile and morphological properties. To better understand skeletal myofiber diversity and plasticity at the transcriptional level, we carried out a whole-genome gene expression analysis of skeletal muscles composed primarily of slow/oxidative fibers (soleus) and fast fibers (extensor digitorum longus, EDL). We also followed gene expression changes in plantaris muscles from mice undergoing voluntary wheel running, a protocol that triggers transformation of glycolytic fibers into oxidative ones. Microarray analysis identified 70 genes differentially expressed by 3-fold or greater in soleus vs. EDL muscles and 15 genes up-regulated in exercised vs. sedentary plantaris muscles. A subset of these results were verified by northern blot and/or real-time RT-PCR analyses. Our results expand knowledge of the differences among various types of skeletal myofibers and their adaptation to exercise at the transcriptional level.
AB - Vertebrate skeletal myofibers are heterogeneous with respect to their metabolic, contractile and morphological properties. To better understand skeletal myofiber diversity and plasticity at the transcriptional level, we carried out a whole-genome gene expression analysis of skeletal muscles composed primarily of slow/oxidative fibers (soleus) and fast fibers (extensor digitorum longus, EDL). We also followed gene expression changes in plantaris muscles from mice undergoing voluntary wheel running, a protocol that triggers transformation of glycolytic fibers into oxidative ones. Microarray analysis identified 70 genes differentially expressed by 3-fold or greater in soleus vs. EDL muscles and 15 genes up-regulated in exercised vs. sedentary plantaris muscles. A subset of these results were verified by northern blot and/or real-time RT-PCR analyses. Our results expand knowledge of the differences among various types of skeletal myofibers and their adaptation to exercise at the transcriptional level.
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U2 - 10.1023/B:JURE.0000009968.60331.86
DO - 10.1023/B:JURE.0000009968.60331.86
M3 - Article
C2 - 14870974
AN - SCOPUS:0942301256
SN - 0142-4319
VL - 24
SP - 587
EP - 592
JO - Journal of Muscle Research and Cell Motility
JF - Journal of Muscle Research and Cell Motility
IS - 8
ER -