TY - JOUR
T1 - Toxoplasma ERK7 protects the apical complex from premature degradation
AU - O’shaughnessy, William J.
AU - Hu, Xiaoyu
AU - Henriquez, Sarah Ana
AU - Reese, Michael L.
N1 - Funding Information:
M.L. Reese acknowledges funding from the Welch Foundation (I-2075-20210327), National Science Foundation (MCB1553334), and National Institutes of Health (AI150715). X. Hu was funded, in part, by Cancer Prevention and Research Institute of Texas Training Grant RP160157. S.A. Henriquez was funded, in part, by a National Science Foundation Graduate Research Fellowship Program.
Publisher Copyright:
© 2023 O’Shaugnessy et al.
PY - 2023
Y1 - 2023
N2 - Accurate cellular replication balances the biogenesis and turnover of complex structures. In the apicomplexan parasite Toxoplasma gondii, daughter cells form within an intact mother cell, creating additional challenges to ensuring fidelity of division. The apical complex is critical to parasite infectivity and consists of apical secretory organelles and specialized cytoskeletal structures. We previously identified the kinase ERK7 as required for maturation of the apical complex in Toxoplasma. Here, we define the Toxoplasma ERK7 interactome, including a putative E3 ligase, CSAR1. Genetic disruption of CSAR1 fully suppresses loss of the apical complex upon ERK7 knockdown. Furthermore, we show that CSAR1 is normally responsible for turnover of maternal cytoskeleton during cytokinesis, and that its aberrant function is driven by mislocalization from the parasite residual body to the apical complex. These data identify a protein homeostasis pathway critical for Toxoplasma replication and fitness and suggest an unappreciated role for the parasite residual body in compartmentalizing processes that threaten the fidelity of parasite development.
AB - Accurate cellular replication balances the biogenesis and turnover of complex structures. In the apicomplexan parasite Toxoplasma gondii, daughter cells form within an intact mother cell, creating additional challenges to ensuring fidelity of division. The apical complex is critical to parasite infectivity and consists of apical secretory organelles and specialized cytoskeletal structures. We previously identified the kinase ERK7 as required for maturation of the apical complex in Toxoplasma. Here, we define the Toxoplasma ERK7 interactome, including a putative E3 ligase, CSAR1. Genetic disruption of CSAR1 fully suppresses loss of the apical complex upon ERK7 knockdown. Furthermore, we show that CSAR1 is normally responsible for turnover of maternal cytoskeleton during cytokinesis, and that its aberrant function is driven by mislocalization from the parasite residual body to the apical complex. These data identify a protein homeostasis pathway critical for Toxoplasma replication and fitness and suggest an unappreciated role for the parasite residual body in compartmentalizing processes that threaten the fidelity of parasite development.
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U2 - 10.1083/jcb.202209098
DO - 10.1083/jcb.202209098
M3 - Article
C2 - 37027006
AN - SCOPUS:85152167600
SN - 0021-9525
VL - 222
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 6
M1 - e202209098
ER -