TY - JOUR
T1 - Toxin-antitoxin selection for isolating somatic cell fusion products between any cell types
AU - Wright, W. E.
PY - 1984
Y1 - 1984
N2 - A selective system was developed that permits the isolation of heterokaryons and cell hybrids between any cell types without the use of selectable genetic markers. Antibodies were introduced into two populations of non-transformed normal diploid human fibroblasts by the osmotic lysis of pinosomes containing hypertonic antibody solutions. Heterokaryons formed by fusing antiricin-injected cells to antidiptheria toxin-injected cells survived an overnight treatment in both ricin and diphtheria toxin. This approach should have general applicability to a variety of investigations such as the complementation analysis of human genetic syndromes, heterokaryon studies of cell differentiation, tumorigenesis and growth control, cell reconstruction experiments, and the production of hybridoma cells.
AB - A selective system was developed that permits the isolation of heterokaryons and cell hybrids between any cell types without the use of selectable genetic markers. Antibodies were introduced into two populations of non-transformed normal diploid human fibroblasts by the osmotic lysis of pinosomes containing hypertonic antibody solutions. Heterokaryons formed by fusing antiricin-injected cells to antidiptheria toxin-injected cells survived an overnight treatment in both ricin and diphtheria toxin. This approach should have general applicability to a variety of investigations such as the complementation analysis of human genetic syndromes, heterokaryon studies of cell differentiation, tumorigenesis and growth control, cell reconstruction experiments, and the production of hybridoma cells.
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U2 - 10.1073/pnas.81.24.7822
DO - 10.1073/pnas.81.24.7822
M3 - Article
C2 - 6595662
AN - SCOPUS:0021711174
SN - 0027-8424
VL - 81
SP - 7822
EP - 7826
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 24 I
ER -