TY - JOUR
T1 - The vast majority of CLA+ T cells are resident in normal skin
AU - Clark, Rachael A.
AU - Chong, Benjamin
AU - Mirchandani, Nina
AU - Brinster, Nooshin K.
AU - Yamanaka, Kei Ichi
AU - Dowgiert, Rebecca K.
AU - Kupper, Thomas S.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2006/4/1
Y1 - 2006/4/1
N2 - There are T cells within normal, noninflamed skin that most likely conduct immunosurveillance and are implicated in the development of psoriasis. We isolated T cells from normal human skin using both established and novel methods. Skin resident T cells expressed high levels of CLA, CCR4, and CCR6, and a subset expressed CCR8 and CXCR6. Skin T cells had a remarkably diverse TCR repertoire and were mostly Th1 memory effector cells with smaller subsets of central memory, Th2, and functional T regulatory cells. We isolated a surprising number of nonexpanded T cells from normal skin. To validate this finding, we counted T cells in sections of normal skin and determined that there are ∼1 × 106 T cells/cm2 normal skin and an estimated 2 × 1010 T cells in the entire skin surface, nearly twice the number of T cells in the circulation. Moreover, we estimate that 98% of CLA + elector memory T cells are resident in normal skin under resting conditions. These findings demonstrate that there is a large pool of memory T cells in normal skin that can initiate and perpetuate immune reactions in the absence of T cell recruitment from the blood.
AB - There are T cells within normal, noninflamed skin that most likely conduct immunosurveillance and are implicated in the development of psoriasis. We isolated T cells from normal human skin using both established and novel methods. Skin resident T cells expressed high levels of CLA, CCR4, and CCR6, and a subset expressed CCR8 and CXCR6. Skin T cells had a remarkably diverse TCR repertoire and were mostly Th1 memory effector cells with smaller subsets of central memory, Th2, and functional T regulatory cells. We isolated a surprising number of nonexpanded T cells from normal skin. To validate this finding, we counted T cells in sections of normal skin and determined that there are ∼1 × 106 T cells/cm2 normal skin and an estimated 2 × 1010 T cells in the entire skin surface, nearly twice the number of T cells in the circulation. Moreover, we estimate that 98% of CLA + elector memory T cells are resident in normal skin under resting conditions. These findings demonstrate that there is a large pool of memory T cells in normal skin that can initiate and perpetuate immune reactions in the absence of T cell recruitment from the blood.
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U2 - 10.4049/jimmunol.176.7.4431
DO - 10.4049/jimmunol.176.7.4431
M3 - Article
C2 - 16547281
AN - SCOPUS:33646046528
SN - 0022-1767
VL - 176
SP - 4431
EP - 4439
JO - Journal of Immunology
JF - Journal of Immunology
IS - 7
ER -