TY - JOUR
T1 - The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins
AU - Gardner, Kevin H.
AU - Kay, Lewis E.
PY - 1998
Y1 - 1998
N2 - During the past thirty years, deuterium labeling has been used to improve the resolution and sensitivity of protein NMR spectra used in a wide variety of applications. Most recently, the combination of triple resonance experiments and 2H, 13C, 15N labeled samples has been critical to the solution structure determination of several proteins with molecular weights on the order of 30 kDa. Here we review the developments in isotopic labeling strategies, NMR pulse sequences, and structure-determination protocols that have facilitated this advance and hold promise for future NMR-based structural studies of even larger systems. As well, we detail recent progress in the use of solution 2H NMR methods to probe the dynamics of protein sidechains.
AB - During the past thirty years, deuterium labeling has been used to improve the resolution and sensitivity of protein NMR spectra used in a wide variety of applications. Most recently, the combination of triple resonance experiments and 2H, 13C, 15N labeled samples has been critical to the solution structure determination of several proteins with molecular weights on the order of 30 kDa. Here we review the developments in isotopic labeling strategies, NMR pulse sequences, and structure-determination protocols that have facilitated this advance and hold promise for future NMR-based structural studies of even larger systems. As well, we detail recent progress in the use of solution 2H NMR methods to probe the dynamics of protein sidechains.
KW - Amino acid-specific isotopic labeling
KW - Protein deuteration
KW - Sidechain dynamics
KW - Structure determination
KW - Triple resonance NMR
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U2 - 10.1146/annurev.biophys.27.1.357
DO - 10.1146/annurev.biophys.27.1.357
M3 - Review article
C2 - 9646872
AN - SCOPUS:0031595590
SN - 1056-8700
VL - 27
SP - 357
EP - 406
JO - Annual Review of Biophysics and Biomolecular Structure
JF - Annual Review of Biophysics and Biomolecular Structure
ER -