The small heat shock protein βA-crystallin negatively regulates pancreatic tumorigenesis

Jifang Liu, Zhongwen Luo, Lan Zhang, Ling Wang, Qian Nie, Zheng Feng Wang, Zhaoxia Huang, Xiaohui Hu, Lili Gong, Andre Patrick Arrigo, Xiangcheng Tang, Jia Wen Xiang, Fangyuan Liu, Mi Deng, Weike Ji, Wenfeng Hu, Ji Ye Zhu, Baojiang Chen, Julia Bridge, Michael A. HollingsworthJames Gigantelli, Yizhi Liu, Quan D. Nguyen, David Wan Cheng Li

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Our recent study has shown that βA-crystallin appears to act as a tumor suppressor in pancreas. Here, we analyzed expression patterns of βA-crystallin in the pancreatic tumor tissue and the neighbor normal tissue from 74 pancreatic cancer patients and also pancreatic cancer cell lines. Immunocytochemistry revealed that βA-crystallin was highly expressed in the normal tissue from 56 patients, but barely detectable in the pancreatic tumor tissue. Moreover, a low level of βAcrystallin predicts poor prognosis for patients with pancreatic duct adenocarcinoma (PDAC). In the 12 pancreatic cell lines analyzed, except for Capan-1 and Miapaca-2 where the level of βA-crystallin was about 80% and 65% of that in the control cell line, HPNE, the remaining pancreatic cancer cells have much lower βA-crystallin levels. Overexpression of βA-crystallin in MiaPaca-1 cells lacking endogenous βAcrystallin significantly decreased its tumorigenicity ability as shown in the colony formation and wound healing assays. In contrast, knockdown of βA-crystallin in the Capan-1 cells significantly increased its tumorigenicity ability as demonstrated in the above assays. Together, our results further demonstrate that βA-crystallin negatively regulates pancreatic tumorigenesis and appears to be a prognosis biomarker for PDAC.

Original languageEnglish (US)
Pages (from-to)65808-65824
Number of pages17
Issue number40
StatePublished - 2016


  • Cancer therapy
  • Pancreatic cancer
  • Small heat shock protein
  • Tumor suppression
  • αA

ASJC Scopus subject areas

  • Oncology


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