TY - JOUR
T1 - The relationship between surface immunoglobulin isotype and immune function of murine b lymphocytes
T2 - I. Surface immunoglobulin isotypes on primed b cells in the spleen*
AU - Zan Bar, I.
AU - Strober, S.
AU - Vitetta, E. S.
PY - 1977/5/1
Y1 - 1977/5/1
N2 - We investigated the ability of IgM-, IgD-, and IgG-bearing cells from the spleens of (BALB/c × C57BL/Ka)F1 mice primed to dinitrophenyl-bovine serum albumin (DNP-BSA) to restore the adoptive secondary anti-BSA and anti-DNP antibody responses. A rabbit anti-mouse IgD antiserum was prepared and the specificity documented by radioimmunoprecipitation, and cell surface staining. Purified populations of IgM-, IgD-, and IgG-bearing cells were prepared by immunofluorescent staining with isotype-specific reagents, and sorting on the fluorescence activated cell sorter. Bright or dull cells were transferred to irradiated syngeneic recipients which were challenged with DNP-BSA in saline. Unfractionated spleen cells restored an adoptive secondary serum antibody response which was all IgG (2-mercaptoethanol resistant). Purified IgM- or IgDbearing cells restored both the secondary IgM and IgG antibody response. IgGbearing cells restored only the IgG response. In addition, the IgG-bearing cells appear to suppress the adoptive secondary IgM response, since depletion of IgGbearing cells from transferred spleen cells results in a marked increase in the adoptive IgM response.
AB - We investigated the ability of IgM-, IgD-, and IgG-bearing cells from the spleens of (BALB/c × C57BL/Ka)F1 mice primed to dinitrophenyl-bovine serum albumin (DNP-BSA) to restore the adoptive secondary anti-BSA and anti-DNP antibody responses. A rabbit anti-mouse IgD antiserum was prepared and the specificity documented by radioimmunoprecipitation, and cell surface staining. Purified populations of IgM-, IgD-, and IgG-bearing cells were prepared by immunofluorescent staining with isotype-specific reagents, and sorting on the fluorescence activated cell sorter. Bright or dull cells were transferred to irradiated syngeneic recipients which were challenged with DNP-BSA in saline. Unfractionated spleen cells restored an adoptive secondary serum antibody response which was all IgG (2-mercaptoethanol resistant). Purified IgM- or IgDbearing cells restored both the secondary IgM and IgG antibody response. IgGbearing cells restored only the IgG response. In addition, the IgG-bearing cells appear to suppress the adoptive secondary IgM response, since depletion of IgGbearing cells from transferred spleen cells results in a marked increase in the adoptive IgM response.
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U2 - 10.1084/jem.145.5.1188
DO - 10.1084/jem.145.5.1188
M3 - Article
C2 - 323402
AN - SCOPUS:0017744957
SN - 0022-1007
VL - 145
SP - 1188
EP - 1205
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 5
ER -