@article{7ea9e99eba6a4e9ba1ee82be65a3f6cf,
title = "The nucleus acts as a ruler tailoring cell responses to spatial constraints",
abstract = "The microscopic environment inside a metazoan organism is highly crowded. Whether individual cells can tailor their behavior to the limited space remains unclear. In this study, we found that cells measure the degree of spatial confinement by using their largest and stiffest organelle, the nucleus. Cell confinement below a resting nucleus size deforms the nucleus, which expands and stretches its envelope. This activates signaling to the actomyosin cortex via nuclear envelope stretch-sensitive proteins, up-regulating cell contractility. We established that the tailored contractile response constitutes a nuclear ruler-based signaling pathway involved in migratory cell behaviors. Cells rely on the nuclear ruler to modulate the motive force that enables their passage through restrictive pores in complex three-dimensional environments, a process relevant to cancer cell invasion, immune responses, and embryonic development.",
author = "Lomakin, {A. J.} and Cattin, {C. J.} and D. Cuvelier and Z. Alraies and M. Molina and Nader, {G. P.F.} and N. Srivastava and Saez, {P. J.} and Garcia-Arcos, {J. M.} and Zhitnyak, {I. Y.} and A. Bhargava and Driscoll, {M. K.} and Welf, {E. S.} and R. Fiolka and Petrie, {R. J.} and {de Silva}, {N. S.} and Gonz{\'a}lez-Granado, {J. M.} and N. Manel and Lennon-Dum{\'e}nil, {A. M.} and M{\"u}ller, {D. J.} and M. Piel",
note = "Funding Information: The research leading to these results has received funding from the People Programme (Marie Sk{\l}odowska-Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement PCOFUND-GA-2013-609102, through the PRESTIGE programme coordinated by Campus France. A.J.L. was supported by the Marie Curie & PRESTIGE Fellowship (grant 609102), London Law Trust Medal Fellowship (grant MGS9403), and a Career Grant for Incoming International Talent (grant 875764) from the Austrian Research Promotion Agency (FFG). D.J.M. was supported by the National Center of Competence in Research (NCCR) Molecular Systems Engineering. This work was also supported by the Institut Pierre-Gilles de Gennes-IPGG (Equipement d'Excellence, “Investissements d'avenir,” program ANR-10-EQPX-34 and Laboratoire d'Excellence, “Investissements d'avenir” program ANR-10-IDEX-0001-02 PSL and ANR-10-LABX-31. This work was additionally supported by the Institut National du Cancer (INCa grant 2018-PL Bio-02) to M.P. and INCa (grant 2019-PL BIO-07) and INSERM Plan Cancer Single Cell (grant 19CS007-00) to N.M. and M.P. R.J.P. was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM126054. R.F. received funding from the National Institutes of Health (grants R35GM133522-01 and R33CA235254-02). J.M.G.G. was financed by Instituto de Salud Carlos III (ISCIII) (PI17/01395; PI20/00306) and I3 SNS program. M.D. was supported by the National Institute of General Medical Sciences (grant K99GM123221). N.S.D.S. received a Marie Sk{\l}odowska-Curie Individual Fellowship (DCBIO 751735) and an EMBO Long-Term Fellowship (ALTF 1298-2016). I.Z. was supported by a Metchnikov Fellowship from the Franco-Russian Scientific Cooperation Program and the Russian Science Foundation (grant 16-15-10288). Publisher Copyright: {\textcopyright} 2020 American Association for the Advancement of Science. All rights reserved.",
year = "2020",
month = oct,
day = "16",
doi = "10.1126/science.aba2894",
language = "English (US)",
volume = "370",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6514",
}