The membrane-associated form of cyclin D1 enhances cellular invasion

Ke Chen; Xuanmao Jiao; Anthony Ashton; Agnese Di Rocco; Timothy G. Pestell; Yunguang Sun; Jun Zhao; Mathew C. Casimiro; Zhiping Li; Michael P. Lisanti; Peter A. McCue; Duanwen Shen; Samuel Achilefu; Hallgeir Rui; Richard G. Pestell

doi:10.1038/s41389-020-00266-y

The membrane-associated form of cyclin D1 enhances cellular invasion

Ke Chen, Xuanmao Jiao, Anthony Ashton, Agnese Di Rocco, Timothy G. Pestell, Yunguang Sun, Jun Zhao, Mathew C. Casimiro, Zhiping Li, Michael P. Lisanti, Peter A. McCue, Duanwen Shen, Samuel Achilefu, Hallgeir Rui, Richard G. Pestell

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The essential G₁-cyclin, CCND1, is a collaborative nuclear oncogene that is frequently overexpressed in cancer. D-type cyclins bind and activate CDK4 and CDK6 thereby contributing to G₁–S cell-cycle progression. In addition to the nucleus, herein cyclin D1 was also located in the cytoplasmic membrane. In contrast with the nuclear-localized form of cyclin D1 (cyclin D1^NL), the cytoplasmic membrane-localized form of cyclin D1 (cyclin D1^MEM) induced transwell migration and the velocity of cellular migration. The cyclin D1^MEM was sufficient to induce G₁–S cell-cycle progression, cellular proliferation, and colony formation. The cyclin D1^MEM was sufficient to induce phosphorylation of the serine threonine kinase Akt (Ser473) and augmented extranuclear localized 17β-estradiol dendrimer conjugate (EDC)-mediated phosphorylation of Akt (Ser473). These studies suggest distinct subcellular compartments of cell cycle proteins may convey distinct functions.

Original language	English (US)
Article number	83
Journal	Oncogenesis
Volume	9
Issue number	9
DOIs	https://doi.org/10.1038/s41389-020-00266-y
State	Published - Sep 1 2020
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Cancer Research

Access to Document

10.1038/s41389-020-00266-y

Cite this

@article{76818ce08adc4cf2a3535e1cf0649f9b,

title = "The membrane-associated form of cyclin D1 enhances cellular invasion",

abstract = "The essential G1-cyclin, CCND1, is a collaborative nuclear oncogene that is frequently overexpressed in cancer. D-type cyclins bind and activate CDK4 and CDK6 thereby contributing to G1–S cell-cycle progression. In addition to the nucleus, herein cyclin D1 was also located in the cytoplasmic membrane. In contrast with the nuclear-localized form of cyclin D1 (cyclin D1NL), the cytoplasmic membrane-localized form of cyclin D1 (cyclin D1MEM) induced transwell migration and the velocity of cellular migration. The cyclin D1MEM was sufficient to induce G1–S cell-cycle progression, cellular proliferation, and colony formation. The cyclin D1MEM was sufficient to induce phosphorylation of the serine threonine kinase Akt (Ser473) and augmented extranuclear localized 17β-estradiol dendrimer conjugate (EDC)-mediated phosphorylation of Akt (Ser473). These studies suggest distinct subcellular compartments of cell cycle proteins may convey distinct functions.",

author = "Ke Chen and Xuanmao Jiao and Anthony Ashton and {Di Rocco}, Agnese and Pestell, {Timothy G.} and Yunguang Sun and Jun Zhao and Casimiro, {Mathew C.} and Zhiping Li and Lisanti, {Michael P.} and McCue, {Peter A.} and Duanwen Shen and Samuel Achilefu and Hallgeir Rui and Pestell, {Richard G.}",

note = "Publisher Copyright: {\textcopyright} 2020, The Author(s).",

year = "2020",

month = sep,

day = "1",

doi = "10.1038/s41389-020-00266-y",

language = "English (US)",

volume = "9",

journal = "Oncogenesis",

issn = "2157-9024",

publisher = "Nature Publishing Group",

number = "9",

}

TY - JOUR

T1 - The membrane-associated form of cyclin D1 enhances cellular invasion

AU - Chen, Ke

AU - Jiao, Xuanmao

AU - Ashton, Anthony

AU - Di Rocco, Agnese

AU - Pestell, Timothy G.

AU - Sun, Yunguang

AU - Zhao, Jun

AU - Casimiro, Mathew C.

AU - Li, Zhiping

AU - Lisanti, Michael P.

AU - McCue, Peter A.

AU - Shen, Duanwen

AU - Achilefu, Samuel

AU - Rui, Hallgeir

AU - Pestell, Richard G.

PY - 2020/9/1

Y1 - 2020/9/1

N2 - The essential G1-cyclin, CCND1, is a collaborative nuclear oncogene that is frequently overexpressed in cancer. D-type cyclins bind and activate CDK4 and CDK6 thereby contributing to G1–S cell-cycle progression. In addition to the nucleus, herein cyclin D1 was also located in the cytoplasmic membrane. In contrast with the nuclear-localized form of cyclin D1 (cyclin D1NL), the cytoplasmic membrane-localized form of cyclin D1 (cyclin D1MEM) induced transwell migration and the velocity of cellular migration. The cyclin D1MEM was sufficient to induce G1–S cell-cycle progression, cellular proliferation, and colony formation. The cyclin D1MEM was sufficient to induce phosphorylation of the serine threonine kinase Akt (Ser473) and augmented extranuclear localized 17β-estradiol dendrimer conjugate (EDC)-mediated phosphorylation of Akt (Ser473). These studies suggest distinct subcellular compartments of cell cycle proteins may convey distinct functions.

AB - The essential G1-cyclin, CCND1, is a collaborative nuclear oncogene that is frequently overexpressed in cancer. D-type cyclins bind and activate CDK4 and CDK6 thereby contributing to G1–S cell-cycle progression. In addition to the nucleus, herein cyclin D1 was also located in the cytoplasmic membrane. In contrast with the nuclear-localized form of cyclin D1 (cyclin D1NL), the cytoplasmic membrane-localized form of cyclin D1 (cyclin D1MEM) induced transwell migration and the velocity of cellular migration. The cyclin D1MEM was sufficient to induce G1–S cell-cycle progression, cellular proliferation, and colony formation. The cyclin D1MEM was sufficient to induce phosphorylation of the serine threonine kinase Akt (Ser473) and augmented extranuclear localized 17β-estradiol dendrimer conjugate (EDC)-mediated phosphorylation of Akt (Ser473). These studies suggest distinct subcellular compartments of cell cycle proteins may convey distinct functions.

UR - http://www.scopus.com/inward/record.url?scp=85091177817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85091177817&partnerID=8YFLogxK

U2 - 10.1038/s41389-020-00266-y

DO - 10.1038/s41389-020-00266-y

M3 - Article

C2 - 32948740

AN - SCOPUS:85091177817

SN - 2157-9024

VL - 9

JO - Oncogenesis

JF - Oncogenesis

IS - 9

M1 - 83

ER -

The membrane-associated form of cyclin D1 enhances cellular invasion

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this