Abstract
The large scale preparation of two second generation immunotoxins containing murine monoclonal antibodies and deglycosylated ricin A chain is described. The procedure for the preparation of immunotoxins consists of the derivatization of antibody with SMPT and reduction of dgA with DTT followed by their reaction to establish a hindered interchain disulfide bond. The purification of the immunotoxin includes affinity chromatography on Blue-Sepharose to remove the free antibody and gel filtration on Sephacryl S-200HR to remove any high molecular weight material and free dgA. The two immunotoxins were prepared by GLP procedures and tested for yield, composition, purity, sterility and biological activity.
Original language | English (US) |
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Pages (from-to) | 223-230 |
Number of pages | 8 |
Journal | Journal of Immunological Methods |
Volume | 142 |
Issue number | 2 |
DOIs | |
State | Published - Sep 13 1991 |
Keywords
- Deglycosylated ricin A chain
- Good laboratory practice
- Immunotoxin
- Large scale
- Monoclonal antibody
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology