The exocyst is a Ral effector complex

Serge Moskalenko; Dale O. Henry; Carine Rosse; Gladys Mirey; Jacques H. Camonis; Michael A. White

doi:10.1038/ncb728

The exocyst is a Ral effector complex

Serge Moskalenko, Dale O. Henry, Carine Rosse, Gladys Mirey, Jacques H. Camonis, Michael A. White

Research output: Contribution to journal › Article › peer-review

358 Scopus citations

Abstract

Delivery of cytoplasmic vesicles to discrete plasma-membrane domains is critical for establishing and maintaining cell polarity, neurite differentiation and regulated exocytosis. The exocyst is a multisubunit complex required for vectorial targeting of a subset of secretory vesicles. Mechanisms that regulate the activity of this complex in mammals are unknown. Here we show that Sec5, an integral component of the exocyst, is a direct target for activated Ral GTPases. Ral GTPases regulate targeting of basolateral proteins in epithelial cells, secretagogue-dependent exocytosis in neuroendocrine cells and assembly of exocyst complexes. These observations define Ral GTPases as critical regulators of vesicle trafficking.

Original language	English (US)
Pages (from-to)	66-72
Number of pages	7
Journal	Nature cell biology
Volume	4
Issue number	1
DOIs	https://doi.org/10.1038/ncb728
State	Published - 2002

ASJC Scopus subject areas

Cell Biology

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title = "The exocyst is a Ral effector complex",

abstract = "Delivery of cytoplasmic vesicles to discrete plasma-membrane domains is critical for establishing and maintaining cell polarity, neurite differentiation and regulated exocytosis. The exocyst is a multisubunit complex required for vectorial targeting of a subset of secretory vesicles. Mechanisms that regulate the activity of this complex in mammals are unknown. Here we show that Sec5, an integral component of the exocyst, is a direct target for activated Ral GTPases. Ral GTPases regulate targeting of basolateral proteins in epithelial cells, secretagogue-dependent exocytosis in neuroendocrine cells and assembly of exocyst complexes. These observations define Ral GTPases as critical regulators of vesicle trafficking.",

author = "Serge Moskalenko and Henry, {Dale O.} and Carine Rosse and Gladys Mirey and Camonis, {Jacques H.} and White, {Michael A.}",

note = "Funding Information: ACKNOWLEDGEMENTS We thank Michael Roth, Karl Matlin, Ira Mellman, Alan Sorkin, Peter Novick and Philip Chavrier for some of the reagents used in these studies. We also thank Michael Roth for many helpful discussions and advice. This work was supported by the National Cancer Institute (CA71443), the Welch foundation (I-1414) and the Association de Recherche sur le Cancer (5440). Correspondence and requests for materials should be addressed to M.A.W.",

year = "2002",

doi = "10.1038/ncb728",

language = "English (US)",

volume = "4",

pages = "66--72",

journal = "Nature cell biology",

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publisher = "Nature Publishing Group",

number = "1",

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TY - JOUR

T1 - The exocyst is a Ral effector complex

AU - Moskalenko, Serge

AU - Henry, Dale O.

AU - Rosse, Carine

AU - Mirey, Gladys

AU - Camonis, Jacques H.

AU - White, Michael A.

N1 - Funding Information: ACKNOWLEDGEMENTS We thank Michael Roth, Karl Matlin, Ira Mellman, Alan Sorkin, Peter Novick and Philip Chavrier for some of the reagents used in these studies. We also thank Michael Roth for many helpful discussions and advice. This work was supported by the National Cancer Institute (CA71443), the Welch foundation (I-1414) and the Association de Recherche sur le Cancer (5440). Correspondence and requests for materials should be addressed to M.A.W.

PY - 2002

Y1 - 2002

N2 - Delivery of cytoplasmic vesicles to discrete plasma-membrane domains is critical for establishing and maintaining cell polarity, neurite differentiation and regulated exocytosis. The exocyst is a multisubunit complex required for vectorial targeting of a subset of secretory vesicles. Mechanisms that regulate the activity of this complex in mammals are unknown. Here we show that Sec5, an integral component of the exocyst, is a direct target for activated Ral GTPases. Ral GTPases regulate targeting of basolateral proteins in epithelial cells, secretagogue-dependent exocytosis in neuroendocrine cells and assembly of exocyst complexes. These observations define Ral GTPases as critical regulators of vesicle trafficking.

AB - Delivery of cytoplasmic vesicles to discrete plasma-membrane domains is critical for establishing and maintaining cell polarity, neurite differentiation and regulated exocytosis. The exocyst is a multisubunit complex required for vectorial targeting of a subset of secretory vesicles. Mechanisms that regulate the activity of this complex in mammals are unknown. Here we show that Sec5, an integral component of the exocyst, is a direct target for activated Ral GTPases. Ral GTPases regulate targeting of basolateral proteins in epithelial cells, secretagogue-dependent exocytosis in neuroendocrine cells and assembly of exocyst complexes. These observations define Ral GTPases as critical regulators of vesicle trafficking.

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The exocyst is a Ral effector complex

Abstract

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