The electron transfer chain of aerobically grown Rhodopseudomonas viridis

Charlotte Kämpf; R. Max Wynn; Robert W. Shaw; David B. Knaff

doi:10.1016/0005-2728(87)90192-7

The electron transfer chain of aerobically grown Rhodopseudomonas viridis

Charlotte Kämpf, R. Max Wynn, Robert W. Shaw, David B. Knaff

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Chemotrophically grown Rhodopseudomonas viridis contains little bacteriochlorophyll b and no detectable reaction center. Membranes isolated from chemotrophically grown Rps. viridis appear to contain cytochrome d and cytochrome o terminal oxidases. The membranes also appear to contain a cytochrome bc₁ complex, since cytochrome c₁ (M_r = 31 000, detected by immunoblotting) and b-cytochromes are present. Chemotrophically grown Rps. viridis contains neither the membrane-bound, reaction center-associated cytochrome c-552 c-558 peptide nor any soluble cytochrome c₂. A previously undetected soluble b-type cytochrome, apparently absent in phototrophically grown Rps. viridis, was found in chemotrophically grown cells. Preliminary measurements indicate that cytochrome b-560 is probably a dimer of about 20 kDa subunits and has an oxidation-reduction midpoint potential of -20 mV.

Original language	English (US)
Pages (from-to)	228-238
Number of pages	11
Journal	BBA - Bioenergetics
Volume	894
Issue number	2
DOIs	https://doi.org/10.1016/0005-2728(87)90192-7
State	Published - Nov 19 1987

Keywords

(Rps. viridis)
Chemotrophic growth
Cytochrome b-560
Electron transfer chain

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2728(87)90192-7

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title = "The electron transfer chain of aerobically grown Rhodopseudomonas viridis",

abstract = "Chemotrophically grown Rhodopseudomonas viridis contains little bacteriochlorophyll b and no detectable reaction center. Membranes isolated from chemotrophically grown Rps. viridis appear to contain cytochrome d and cytochrome o terminal oxidases. The membranes also appear to contain a cytochrome bc1 complex, since cytochrome c1 (Mr = 31 000, detected by immunoblotting) and b-cytochromes are present. Chemotrophically grown Rps. viridis contains neither the membrane-bound, reaction center-associated cytochrome c-552 c-558 peptide nor any soluble cytochrome c2. A previously undetected soluble b-type cytochrome, apparently absent in phototrophically grown Rps. viridis, was found in chemotrophically grown cells. Preliminary measurements indicate that cytochrome b-560 is probably a dimer of about 20 kDa subunits and has an oxidation-reduction midpoint potential of -20 mV.",

keywords = "(Rps. viridis), Chemotrophic growth, Cytochrome b-560, Electron transfer chain",

author = "Charlotte K{\"a}mpf and Wynn, {R. Max} and Shaw, {Robert W.} and Knaff, {David B.}",

note = "Funding Information: The authors would like to thank Professors Chang-An Yu and Samuel Kaplan for generous gifts of antibodies, Professor Norbert Pfennig for the gift of the Rps. oiridis culture, Professor K. John Morrow, Jr. and Mr. Kevin A. Gray for assistance with some of the immunological assays and Mr. Aidas Kriauciunas for help in growing the Rps. viridis cells used for some of the experiments. The authors also wish to thank Dr. Roger Prince and Dr. Fevzi Daldal for helpful discussions. This work was supported by a grant (to D.B.K.) from the National Science Foundation (PCM 84-08564) and (to R.W.S.) from the Robert A. Welch Foundation (D-909).",

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doi = "10.1016/0005-2728(87)90192-7",

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T1 - The electron transfer chain of aerobically grown Rhodopseudomonas viridis

AU - Kämpf, Charlotte

AU - Wynn, R. Max

AU - Shaw, Robert W.

AU - Knaff, David B.

N1 - Funding Information: The authors would like to thank Professors Chang-An Yu and Samuel Kaplan for generous gifts of antibodies, Professor Norbert Pfennig for the gift of the Rps. oiridis culture, Professor K. John Morrow, Jr. and Mr. Kevin A. Gray for assistance with some of the immunological assays and Mr. Aidas Kriauciunas for help in growing the Rps. viridis cells used for some of the experiments. The authors also wish to thank Dr. Roger Prince and Dr. Fevzi Daldal for helpful discussions. This work was supported by a grant (to D.B.K.) from the National Science Foundation (PCM 84-08564) and (to R.W.S.) from the Robert A. Welch Foundation (D-909).

PY - 1987/11/19

Y1 - 1987/11/19

N2 - Chemotrophically grown Rhodopseudomonas viridis contains little bacteriochlorophyll b and no detectable reaction center. Membranes isolated from chemotrophically grown Rps. viridis appear to contain cytochrome d and cytochrome o terminal oxidases. The membranes also appear to contain a cytochrome bc1 complex, since cytochrome c1 (Mr = 31 000, detected by immunoblotting) and b-cytochromes are present. Chemotrophically grown Rps. viridis contains neither the membrane-bound, reaction center-associated cytochrome c-552 c-558 peptide nor any soluble cytochrome c2. A previously undetected soluble b-type cytochrome, apparently absent in phototrophically grown Rps. viridis, was found in chemotrophically grown cells. Preliminary measurements indicate that cytochrome b-560 is probably a dimer of about 20 kDa subunits and has an oxidation-reduction midpoint potential of -20 mV.

AB - Chemotrophically grown Rhodopseudomonas viridis contains little bacteriochlorophyll b and no detectable reaction center. Membranes isolated from chemotrophically grown Rps. viridis appear to contain cytochrome d and cytochrome o terminal oxidases. The membranes also appear to contain a cytochrome bc1 complex, since cytochrome c1 (Mr = 31 000, detected by immunoblotting) and b-cytochromes are present. Chemotrophically grown Rps. viridis contains neither the membrane-bound, reaction center-associated cytochrome c-552 c-558 peptide nor any soluble cytochrome c2. A previously undetected soluble b-type cytochrome, apparently absent in phototrophically grown Rps. viridis, was found in chemotrophically grown cells. Preliminary measurements indicate that cytochrome b-560 is probably a dimer of about 20 kDa subunits and has an oxidation-reduction midpoint potential of -20 mV.

KW - (Rps. viridis)

KW - Chemotrophic growth

KW - Cytochrome b-560

KW - Electron transfer chain

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The electron transfer chain of aerobically grown Rhodopseudomonas viridis

Abstract

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