The effects of nitrous oxide on the central endogenous pro-opiomelanocortin system in the rat

John R Zuniga; Shirley A. Joseph; Karl M. Knigge

doi:10.1016/0006-8993(87)90239-3

The effects of nitrous oxide on the central endogenous pro-opiomelanocortin system in the rat

John R Zuniga, Shirley A. Joseph, Karl M. Knigge

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The hypothesis that nitrous oxide stimulates the central pro-opiomelanocortin system in vivo was explored in this study. A concentration-dependent stimulation of central pro-opiomelanocortin neuropeptides was demonstrated after exposures to variable concentrations of nitrous oxide with oxygen. Rats exposed to 60% and 80% nitrous oxide with oxygen demonstrated an elevation of β-endorphin concentration along the neuraxis involved with analgesia; no similar effect was observed in α-MSH concentration, neither duration of exposure nor acclimation to the enclosed environment altered this stimulation. The discontinuation of nitrous oxide exposure resulted in the diminution of β-endorphin concentration to pre-exposure levels in 15-30 min. With an ACTH_1-39 antisera, a semi-quantitative increase in opiocortin immunoreactivity after exposures to nitrous oxide was demonstrated. In conclusion, the increase in β-endorphin concentration and immunoreactive ACTH_1-39 staining in the cells of origin, areas of fiber projection and terminal fields suggest that nitrous oxide stimulates the central pro-opiomelanocortin system in vivo in the rat.

Original language	English (US)
Pages (from-to)	57-65
Number of pages	9
Journal	Brain Research
Volume	420
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(87)90239-3
State	Published - Sep 8 1987

Keywords

Adrenocorticotropic hormone
Medial basal hypothalamus
Nitrous oxide
Periaqueductal gray
α-Melanocyte stimulating hormone
β-Endorphin

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(87)90239-3

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title = "The effects of nitrous oxide on the central endogenous pro-opiomelanocortin system in the rat",

abstract = "The hypothesis that nitrous oxide stimulates the central pro-opiomelanocortin system in vivo was explored in this study. A concentration-dependent stimulation of central pro-opiomelanocortin neuropeptides was demonstrated after exposures to variable concentrations of nitrous oxide with oxygen. Rats exposed to 60% and 80% nitrous oxide with oxygen demonstrated an elevation of β-endorphin concentration along the neuraxis involved with analgesia; no similar effect was observed in α-MSH concentration, neither duration of exposure nor acclimation to the enclosed environment altered this stimulation. The discontinuation of nitrous oxide exposure resulted in the diminution of β-endorphin concentration to pre-exposure levels in 15-30 min. With an ACTH1-39 antisera, a semi-quantitative increase in opiocortin immunoreactivity after exposures to nitrous oxide was demonstrated. In conclusion, the increase in β-endorphin concentration and immunoreactive ACTH1-39 staining in the cells of origin, areas of fiber projection and terminal fields suggest that nitrous oxide stimulates the central pro-opiomelanocortin system in vivo in the rat.",

keywords = "Adrenocorticotropic hormone, Medial basal hypothalamus, Nitrous oxide, Periaqueductal gray, α-Melanocyte stimulating hormone, β-Endorphin",

author = "Zuniga, {John R} and Joseph, {Shirley A.} and Knigge, {Karl M.}",

note = "Funding Information: This researchw as supportedin part by the Eastman Dental Center FoundationT eacherTraining Grant and the AmericanA ssociationo f Oral and MaxillofaciaSl urgeryR esearchFe llowshipA ward.",

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AU - Joseph, Shirley A.

AU - Knigge, Karl M.

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N2 - The hypothesis that nitrous oxide stimulates the central pro-opiomelanocortin system in vivo was explored in this study. A concentration-dependent stimulation of central pro-opiomelanocortin neuropeptides was demonstrated after exposures to variable concentrations of nitrous oxide with oxygen. Rats exposed to 60% and 80% nitrous oxide with oxygen demonstrated an elevation of β-endorphin concentration along the neuraxis involved with analgesia; no similar effect was observed in α-MSH concentration, neither duration of exposure nor acclimation to the enclosed environment altered this stimulation. The discontinuation of nitrous oxide exposure resulted in the diminution of β-endorphin concentration to pre-exposure levels in 15-30 min. With an ACTH1-39 antisera, a semi-quantitative increase in opiocortin immunoreactivity after exposures to nitrous oxide was demonstrated. In conclusion, the increase in β-endorphin concentration and immunoreactive ACTH1-39 staining in the cells of origin, areas of fiber projection and terminal fields suggest that nitrous oxide stimulates the central pro-opiomelanocortin system in vivo in the rat.

AB - The hypothesis that nitrous oxide stimulates the central pro-opiomelanocortin system in vivo was explored in this study. A concentration-dependent stimulation of central pro-opiomelanocortin neuropeptides was demonstrated after exposures to variable concentrations of nitrous oxide with oxygen. Rats exposed to 60% and 80% nitrous oxide with oxygen demonstrated an elevation of β-endorphin concentration along the neuraxis involved with analgesia; no similar effect was observed in α-MSH concentration, neither duration of exposure nor acclimation to the enclosed environment altered this stimulation. The discontinuation of nitrous oxide exposure resulted in the diminution of β-endorphin concentration to pre-exposure levels in 15-30 min. With an ACTH1-39 antisera, a semi-quantitative increase in opiocortin immunoreactivity after exposures to nitrous oxide was demonstrated. In conclusion, the increase in β-endorphin concentration and immunoreactive ACTH1-39 staining in the cells of origin, areas of fiber projection and terminal fields suggest that nitrous oxide stimulates the central pro-opiomelanocortin system in vivo in the rat.

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The effects of nitrous oxide on the central endogenous pro-opiomelanocortin system in the rat

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