TY - JOUR
T1 - The effect of polypeptide hormones on lipid monolayers. II. The effect of insulin analogues, vasopressin, oxytocin, thyrocalcitonin, adrenocorticotropin, and 3′,5′-cyclic AMP on the uptake of Ca2+ by monomolecular films of monooctadecyl phosphate
AU - Kafka, Marian S.
AU - Pak, Charles Y C
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1969/10/14
Y1 - 1969/10/14
N2 - 1. 1. Like insulin, some analogues of insulin inhibited the adsorption of Ca2+ at a monooctadecyl phosphate monolayer; the inhibition was, in all cases, less than the inhibition by intact insulin. 2. 2. Facilitation of release of Ca2+ from the monolayer by insulin required that the insulin molecule be intact, as release was facilitated only very slightly when des-A1-glycine-des-B1-phenylalanine1-insulin or insulin B-chain-S-sulfonate replaced insulin, and did not occur at all in the absence of alanine in position B30. 3. 3. Vasopressin and oxytocin inhibited Ca2+ adsorption. In addition, vasopressin facilitated Ca2+ release. 4. 4. Thyrocalcitonin profoundly inhibited Ca2+ adsorption but facilitated only slightly its release. 5. 5. 3′,5′-cyclic AMP facilitated slightly the adsorption of Ca2+. 6. 6. It is suggested that the biphasic action of insulin and vasopressin on Ca2+ uptake depends upon the concurrence of a 6-membered disulfide ring at or near the N-terminus of the molecule, and a proline followed by a positively charged amino acid (arginine or lysine) in the two positions preceding the C-terminal amino acid.
AB - 1. 1. Like insulin, some analogues of insulin inhibited the adsorption of Ca2+ at a monooctadecyl phosphate monolayer; the inhibition was, in all cases, less than the inhibition by intact insulin. 2. 2. Facilitation of release of Ca2+ from the monolayer by insulin required that the insulin molecule be intact, as release was facilitated only very slightly when des-A1-glycine-des-B1-phenylalanine1-insulin or insulin B-chain-S-sulfonate replaced insulin, and did not occur at all in the absence of alanine in position B30. 3. 3. Vasopressin and oxytocin inhibited Ca2+ adsorption. In addition, vasopressin facilitated Ca2+ release. 4. 4. Thyrocalcitonin profoundly inhibited Ca2+ adsorption but facilitated only slightly its release. 5. 5. 3′,5′-cyclic AMP facilitated slightly the adsorption of Ca2+. 6. 6. It is suggested that the biphasic action of insulin and vasopressin on Ca2+ uptake depends upon the concurrence of a 6-membered disulfide ring at or near the N-terminus of the molecule, and a proline followed by a positively charged amino acid (arginine or lysine) in the two positions preceding the C-terminal amino acid.
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U2 - 10.1016/0005-2736(69)90064-9
DO - 10.1016/0005-2736(69)90064-9
M3 - Article
C2 - 4310719
AN - SCOPUS:0014675029
SN - 0005-2736
VL - 193
SP - 117
EP - 123
JO - BBA - Biomembranes
JF - BBA - Biomembranes
IS - 1
ER -