The effect of insulin and glucose on fructose-2,6-P2 in hepatocytes

Carolyn Sue Richards, Kosaku Uyeda

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


The effect of insulin and glucose on fructose-2,6-P2 turnover has been examined using isolated rat hepatocytes. Insulin (>10-10M) increases the fructose-2,6-P2 level in hepatocytes from fasted rats, or those pretreated with glucagon (<10-10M) but not those from fed rats. Glucose (>10mM) also increases the fructose-2,6-P2 and hexose-P levels in hepatocytes from starved rats, and its effect appears to be greater than that of insulin. Furthermore, insulin increases fructose-6-P,2-kinase activity and decreases cAMP levels in hepatocytes pretreated with glucagon, and it increases hexose-P concentration in hepatocytes from fasted rats. These results suggest that insulin antagonizes glucagon action by increasing fructose-6-P,2-kinase, decreasing cyclic AMP levels, and increasing hexose-P levels, resulting in increased concentrations of fructose-2,6-P2.

Original languageEnglish (US)
Pages (from-to)394-401
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Nov 30 1982

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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