The effect of glutamate on ghrelin release in mice

Rakhi Chacrabati, Zhi Gong, Chika Ikenoya, Daisuke Kondo, Jeffrey M. Zigman, Takafumi Sakai, Ichiro Sakata

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Ghrelin is abundantly produced in the stomach. Here, we found that glutamate decreased ghrelin expression and release in ghrelin-producing cells, and decreased levels of food intake and plasma acyl-ghrelin in mice. Treatment with siRNA of G protein-coupled receptor, family C, group 5, member B (GPRC5B) in ghrelin-producing cell lines completely blocked the effect of glutamate-induced ghrelin suppression. In addition, glutamate inhibited ghrelin release via the extracellular signal-regulated kinase (ERK) activity pathway, and stimulated CREB2 mRNA expression in ghrelin-producing cell lines. These results suggest that glutamate inhibits ghrelin release via ERK-CREB2 pathway. These results suggest that the GPRC5B-ERK-CREB2 pathway is involved in the inhibition of ghrelin expression and secretion in ghrelin cells.

Original languageEnglish (US)
Pages (from-to)320-327
Number of pages8
JournalCell Biology International
Issue number3
StatePublished - Mar 1 2017


  • CREB2
  • GPRC5B
  • extracellular signal-regulated kinase (ERK)
  • ghrelin
  • glutamate

ASJC Scopus subject areas

  • Cell Biology


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