The effect of glutamate on ghrelin release in mice

Rakhi Chacrabati; Zhi Gong; Chika Ikenoya; Daisuke Kondo; Jeffrey M. Zigman; Takafumi Sakai; Ichiro Sakata

doi:10.1002/cbin.10728

The effect of glutamate on ghrelin release in mice

Rakhi Chacrabati, Zhi Gong, Chika Ikenoya, Daisuke Kondo, Jeffrey M. Zigman, Takafumi Sakai, Ichiro Sakata

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Ghrelin is abundantly produced in the stomach. Here, we found that glutamate decreased ghrelin expression and release in ghrelin-producing cells, and decreased levels of food intake and plasma acyl-ghrelin in mice. Treatment with siRNA of G protein-coupled receptor, family C, group 5, member B (GPRC5B) in ghrelin-producing cell lines completely blocked the effect of glutamate-induced ghrelin suppression. In addition, glutamate inhibited ghrelin release via the extracellular signal-regulated kinase (ERK) activity pathway, and stimulated CREB2 mRNA expression in ghrelin-producing cell lines. These results suggest that glutamate inhibits ghrelin release via ERK-CREB2 pathway. These results suggest that the GPRC5B-ERK-CREB2 pathway is involved in the inhibition of ghrelin expression and secretion in ghrelin cells.

Original language	English (US)
Pages (from-to)	320-327
Number of pages	8
Journal	Cell Biology International
Volume	41
Issue number	3
DOIs	https://doi.org/10.1002/cbin.10728
State	Published - Mar 1 2017

Keywords

CREB2
GPRC5B
extracellular signal-regulated kinase (ERK)
ghrelin
glutamate

ASJC Scopus subject areas

Cell Biology

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10.1002/cbin.10728

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title = "The effect of glutamate on ghrelin release in mice",

abstract = "Ghrelin is abundantly produced in the stomach. Here, we found that glutamate decreased ghrelin expression and release in ghrelin-producing cells, and decreased levels of food intake and plasma acyl-ghrelin in mice. Treatment with siRNA of G protein-coupled receptor, family C, group 5, member B (GPRC5B) in ghrelin-producing cell lines completely blocked the effect of glutamate-induced ghrelin suppression. In addition, glutamate inhibited ghrelin release via the extracellular signal-regulated kinase (ERK) activity pathway, and stimulated CREB2 mRNA expression in ghrelin-producing cell lines. These results suggest that glutamate inhibits ghrelin release via ERK-CREB2 pathway. These results suggest that the GPRC5B-ERK-CREB2 pathway is involved in the inhibition of ghrelin expression and secretion in ghrelin cells.",

keywords = "CREB2, GPRC5B, extracellular signal-regulated kinase (ERK), ghrelin, glutamate",

author = "Rakhi Chacrabati and Zhi Gong and Chika Ikenoya and Daisuke Kondo and Zigman, {Jeffrey M.} and Takafumi Sakai and Ichiro Sakata",

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doi = "10.1002/cbin.10728",

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AU - Chacrabati, Rakhi

AU - Gong, Zhi

AU - Ikenoya, Chika

AU - Kondo, Daisuke

AU - Zigman, Jeffrey M.

AU - Sakai, Takafumi

AU - Sakata, Ichiro

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Ghrelin is abundantly produced in the stomach. Here, we found that glutamate decreased ghrelin expression and release in ghrelin-producing cells, and decreased levels of food intake and plasma acyl-ghrelin in mice. Treatment with siRNA of G protein-coupled receptor, family C, group 5, member B (GPRC5B) in ghrelin-producing cell lines completely blocked the effect of glutamate-induced ghrelin suppression. In addition, glutamate inhibited ghrelin release via the extracellular signal-regulated kinase (ERK) activity pathway, and stimulated CREB2 mRNA expression in ghrelin-producing cell lines. These results suggest that glutamate inhibits ghrelin release via ERK-CREB2 pathway. These results suggest that the GPRC5B-ERK-CREB2 pathway is involved in the inhibition of ghrelin expression and secretion in ghrelin cells.

AB - Ghrelin is abundantly produced in the stomach. Here, we found that glutamate decreased ghrelin expression and release in ghrelin-producing cells, and decreased levels of food intake and plasma acyl-ghrelin in mice. Treatment with siRNA of G protein-coupled receptor, family C, group 5, member B (GPRC5B) in ghrelin-producing cell lines completely blocked the effect of glutamate-induced ghrelin suppression. In addition, glutamate inhibited ghrelin release via the extracellular signal-regulated kinase (ERK) activity pathway, and stimulated CREB2 mRNA expression in ghrelin-producing cell lines. These results suggest that glutamate inhibits ghrelin release via ERK-CREB2 pathway. These results suggest that the GPRC5B-ERK-CREB2 pathway is involved in the inhibition of ghrelin expression and secretion in ghrelin cells.

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The effect of glutamate on ghrelin release in mice

Abstract

Keywords

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